60Co-γ射线对2种狼尾草属牧草的诱变效应

武炳超,童磊,杜昭昌,胡家菱,张欢,陈燚,刘伟,张新全,黄琳凯

Mutagenic Effects of 60Co-γ-rays on Two Species of Pennisetum Genus Forages

WU BingChao,TONG Lei,DU ZhaoChang,HU JiaLing,ZHANG Huan,CHEN Yi,LIU Wei,ZHANG XinQuan,HUANG LinKai

表4 “华南” 20对SSR引物序列及其扩增结果

Table 4 20 SSR primers and their amplification results of P. purpureum Schum. cv. Huanan

引物名称 上游引物 下游引物 退火温度 扩增条带数 多态性条带数 多态性条带比率 多态性信息量 Primer name Forward primer Reverse primer Annealing Total number of amplified bands The number of polymorphic bands Percentage of polymorphic bands (%) PIC (5′-3′) (5′-3′) (℃) (%) SSR1 AGCATCGATAGCTTTTTCCTGC GAGGGGAGGAGAGGATGCTT 62 4 3 75 58 SSR3 GTCGCACTGCAATCCAACATA TCCATTCATTCATTCTTCAGTGATTT 62 3 3 100 55.3 SSR9 CGTACCTTCTCGCAATAAGGAGAT GAAGAAGGTTCACCTCTTTCGGTA 62 3 2 66.67 47.7 SSR11 AGAGGAGACAAAGGACCTCCG GTCCATGGAGGACGAGCTTC 62 8 7 87.5 28.6 SSR12 GTCGAGGAAGTCCTTGGTGTG GACGAGGGGTTCATCCACAT 62 4 4 100 58.5 SSR19 CTCTCTTTCTCTCTCTGTTGCGTG CACCACACTGCTCCACCACT 62 6 2 33.33 13.3 SSR23 AGGTTCTGCTTCTGGAGGTGAC TAGATTTGTTCATGATGATGCGTG 62 3 2 66.67 56.1 SSR24 ACCACCATTCCTTCAATTCGTACT GAGAAGTGAGTGCTACAGGTGCAT 62 4 2 50 18.7 SSR25 ATTTCTCTGGTTTTGTTCAGCTCC ACAGAGGTGGTATGATCGACTTGA 62 4 4 100 68.5 SSR29 GCTTTCAAGTGATACAACGACACC CCACTCGTCCTTCAATCTCTACCT 62 4 3 75 10.8 SSR35 ATGTGTTCGTTGCCATCTGTAGTT GCACGGCAAACAAACAAAAA 62 2 2 100 65.9 SSR38 TCTTTAGATCTCTGCCATGTAGCG GCTAGCACTACCCCTGCATTTTAT 62 3 1 33.33 18 SSR43 GTTTCTTGCAAGTTACTCGCTTCA CGTTAGTTCCTCCATTCTCTTTTGA 62 7 7 100 49.1 SSR47 CAATGAAACTATGCACTTAAGCCG AGTCTGTTTTGAGTCGAGGGAATC 62 5 5 100 61.5 SSR50 GCATCAGGAACCATATATGTTGGA AACACAATCGCTAGCTGATCCATA 62 4 3 75 35.3 SSR53 AAATGAAAGTGAAAGGGGAAAACA TTTTTCTTCTGTTATTATTCTCGTGTCC 62 2 2 100 76.5 SSR54 GAAAACTCACCGGAAGGTCAAC GAGGAGAAGACGAACGGTGACT 62 4 3 75 27.4 SSR55 AAGTCCACATCATCCCGGTC GTGGCTTACCTGATCCGAGC 62 4 4 100 27 SSR59 CTCTAATCTCTCTCCTCCCGCTC CTGCTCACCATCAGCAGTGAG 60 4 4 100 7.8 SSR66 ATAATGGAAAGTGAGTTTTGCCGT GGAGCCTTCAGCTTTAAAAGACAA 62 3 2 66.67 4.2 总计Total 81 65 平均Average 4.1 3.3 39.4