60Co-γ射线对2种狼尾草属牧草的诱变效应

武炳超,童磊,杜昭昌,胡家菱,张欢,陈燚,刘伟,张新全,黄琳凯

Mutagenic Effects of 60Co-γ-rays on Two Species of Pennisetum Genus Forages

WU BingChao,TONG Lei,DU ZhaoChang,HU JiaLing,ZHANG Huan,CHEN Yi,LIU Wei,ZHANG XinQuan,HUANG LinKai

表3 “热研4号” 20对SSR引物序列及其扩增结果

Table 3 20 SSR primers and their amplification results of P. purpureum × P. americanurn. cv. ReyanNo.4

引物名称 上游引物 下游引物 退火温度 扩增条带数 多态性条带数 多态性条带比率 多态性信息量 Primer name Forward primer Reverse primer Annealing Total number of amplified bands The number of polymorphic bands Percentage of polymorphic bands (%) PIC (5′-3′) (5′-3′) (℃) (%) SSR8 TCCCTCTAATCACAAATGAGTCCA TTTGGACTGTTTCCCTTTCAAATAA 62 3 3 100 28.5 SSR14 GAGAAGATCAGAGGAGGGTAGCAG CGATGAATGCAATGCAAGCTA 62 3 2 66.67 17.5 SSR16 ACTAGAACTACAGGAGGTCGGGG ATATGGGATGTGTTGAGGTGTGG 62 3 3 100 47.7 SSR19 CTCTCTTTCTCTCTCTGTTGCGTG CACCACACTGCTCCACCACT 62 6 6 100 29.9 SSR24 ACCACCATTCCTTCAATTCGTACT GAGAAGTGAGTGCTACAGGTGCAT 62 2 0 0 0 SSR33 AGGCTCAAGCTCTACGTGGC GACAAACGGCAAACATTTTCAA 58 6 6 100 18.6 SSR39 GACCTGGGGTAGGTCTGATCC CGCTTTGTTTTGGTGTAGATTGAG 62 7 6 85.71 43 SSR42 TTTAGAGAACTCCTACGGTACGGC ATCCACCTAGACACCGTACGAAAT 62 2 1 50 23.2 SSR43 GTTTCTTGCAAGTTACTCGCTTCA CGTTAGTTCCTCCATTCTCTTTTGA 62 3 3 100 75.5 SSR46 CGCAACCAGCATCTCTTCAG CACTGTCGTAGCTCAAGAAGTCGT 62 6 4 66.67 24 SSR47 CAATGAAACTATGCACTTAAGCCG AGTCTGTTTTGAGTCGAGGGAATC 62 4 2 50 18.4 SSR50 GCATCAGGAACCATATATGTTGGA AACACAATCGCTAGCTGATCCATA 62 5 2 40 21.9 SSR51 GTTTGCTTCAACATAACGCAAAAG GATCTGGTGGCTCCTGACTTG 58 3 1 33.33 18 SSR52 AAAGAGCAAAGGCTTTATTTGCAC CGACACAGAGGGAGTGTTATATTTTG 62 4 1 25 50.8 SSR53 AAATGAAAGTGAAAGGGGAAAACA TTTTTCTTCTGTTATTATTCTCGTGTCC 62 3 0 0 0 SSR54 GAAAACTCACCGGAAGGTCAAC GAGGAGAAGACGAACGGTGACT 62 4 3 75 9.3 SSR55 AAGTCCACATCATCCCGGTC GTGGCTTACCTGATCCGAGC 62 8 2 25 7.9 SSR59 CTCTAATCTCTCTCCTCCCGCTC CTGCTCACCATCAGCAGTGAG 60 5 3 60 26.7 SSR65 GCCTGCCTATTGCCTAGACG AAGGAAGATGTGACTCACGGATTT 62 3 3 100 29.2 SSR70 AGCTGATCTTATTTGGCTACTGCC TGTAAAAGCCTACAAGGATGCGTA 62 3 0 0 0 总计Total 83 52 平均Average 4.2 2.6 24.5