液相色谱-串联质谱法同时测定饲用血液制品中 18种β-受体激动剂

索德成,魏书林,肖志明,王培龙,王瑞国,李阳

Simultaneous Determination of 18 β-agonists in Blood Products for Feeds by Liquid Chromatography Tandem Mass Spectrometry

DeCheng SUO,ShuLin WEI,ZhiMing XIAO,PeiLong WANG,RuiGuo WANG,Yang LI

表4 饲用血液制品回收率与精密度实验结果(n=6)

Table 4 Recovery and precision of blood products

添加浓度 Added concentration (μg·kg-1) 药物名称 Drug 血粉Blood meal 血球蛋白粉Haemoglobin 血浆蛋白粉Plasma protein 批内回收率% ±相对标准偏差 Intra recovery ±RSD (%) 批间变异系数 Inter Coefficient of variation (%) 批内回收率% ±相对标准偏差 Intra recovery ±RSD (%) 批间变异系数 Inter Coefficient of variation (%) 批内回收率%±相对标准偏差 Intra recovery ±RSD（%） 批间变异系数 Inter Coefficient of variation (%) 5 沙丁胺醇Salbutamol 82.0±7.8 9.5 85.7±12 14.2 83.0±12 16.6 克仑特罗Clenbuterol 88.2±6.2 13.9 98.5±14.1 14.4 83.0±12.3 18.0 莱克多巴胺Ractopamine 71.0±6.9 9.3 90.7±17.3 8.8 70.7±6.8 7.7 苯乙醇胺A Phenylethanolamine A 70.5±13.4 15.4 102.3±13 13.5 85.2±8.8 8.6 溴布特罗Brombuterol 70.0±13.8 17.4 106.5±12.9 22.6 82.5±8.7 12.2 氯丙那林Clorprenaline 76.9±12.2 13.5 101.2±5.8 16.6 71.2±8.3 14.2 特布他林Terbutaline 76.1±14.0 15.9 111.9±5.7 7.4 79.6±12.1 15.7 齐帕特罗Zilpaterol 80.8±10.1 15.3 94.4±18.8 12.0 83.2±5.8 15.9 马布特罗Mabuterol 71.9±16.3 15.4 95.4±16 12.8 78.3±13.6 15.8 西马特罗Cimaterol 71.7±15.1 16.5 78.1±13.6 14.8 77.3±13 15.9 班布特罗Bambuterol 84.1±10.8 14.6 85.3±8.2 14.0 80.7±13.9 16.7 妥布特罗Tulobuterol 70.4±14.0 16.8 79.2±10.8 14.3 78.6±18.5 19.6 克仑普罗Clenproperol 91.2±13.6 14.7 89.9±14.4 15.6 73.1±11.3 15.4 福莫特罗Formoterol 88.9±10.7 13.7 92.4±9.1 4.3 78.1±10.8 13.9 西布特罗Cimbuterol 79.9±7.6 13.6 82.6±6.5 14.3 88.2±15.5 17.3 沙美特罗Salmeterol 73.4±7.9 10.2 81.1±8.4 8.7 73.2±7.2 8.9 利托君Ritodrine 79.2±9.7 13.3 100.2±11.6 13.4 80.1±6 9.0 克伦塞罗Clencyclohexerol 65.6±2.7 4.1 103.2±13.6 15.9 100.1±6.9 16.1 10 沙丁胺醇Salbutamol 77.0±11.7 13.7 113.3±14.8 2.9 78.6±8.8 11.5 克仑特罗Clenbuterol 78.9±5.8 9.9 91.1±15.7 16.6 85.6±7.8 10.6 莱克多巴胺Ractopamine 76.6±11.5 15.6 90.2±11 17.2 117.8±10.2 12.4 苯乙醇胺A Phenylethanolamine A 86.7±5.0 14.2 91.5±12.5 11.9 81.8±10.6 13.6 溴布特罗Brombuterol 74.6±10.2 15.0 73.7±8.1 12.9 77.8±13.1 12.2 氯丙那林Clorprenaline 82.4±7.0 13.5 70.1±7.3 14.5 83.0±12 13.7 特布他林Terbutaline 73.3±13.8 13.2 79.6±13.5 13.7 25.8±3.1 8.0 齐帕特罗Zilpaterol 70.2±7.7 15.5 102.1±12.2 13.7 72.8±12.5 12.6 马布特罗Mabuterol 76.6±2.3 5.6 107.7±3.3 15.7 73.3±11 12.6 西马特罗Cimaterol 86.6±9.7 12.1 102.3±6.5 14.2 107.8±11.3 17.3 班布特罗Bambuterol 84.0±6.2 12.8 107.9±15.4 14.6 120.8±7.2 12.7 妥布特罗Tulobuterol 90.3±4.3 8.5 81.7±7.5 9.3 87.0±12.8 13.9 克仑普罗Clenproperol 89.2±11.7 13.3 98.4±7.6 13.4 85.2±3.1 3.4 福莫特罗Formoterol 77.3±4.0 9.0 100.0±13.5 17.1 81.8±12.6 12.2 西布特罗Cimbuterol 85.6±8.1 4.8 72.5±14 16.3 70.1±11.2 13.3 沙美特罗Salmeterol 79.9±6.3 11.3 84.4±13.4 6.8 77.6±7.3 10.2 利托君Ritodrine 77.7±9.2 4.4 102.7±9.7 11.6 79.4±13.7 8.6 克伦塞罗Clencyclohexerol 87.5±9.5 10.4 85.6±13.1 9.6 99.2±6.3 14.0 50 沙丁胺醇Salbutamol 97.0±9.6 15.8 110.0±13.5 12.0 78.1±7.8 12.7 克仑特罗Clenbuterol 86.8±4.3 10.4 102.5±17 14.0 74.2±10.6 12.5 莱克多巴胺Ractopamine 97.2±10.2 13.1 102.2±12.2 13.9 79.2±7.7 9.1 苯乙醇胺A Phenylethanolamine A 89.9±4.2 4.6 107.7±3.3 13.6 74.0±6.8 9.0 溴布特罗Brombuterol 93.3±13.5 15.5 88.9±13.1 16.0 88.8±6.2 8.7 氯丙那林Clorprenaline 91.6±5.0 10.7 77.6±8.7 4.5 81.5±8.2 9.2 特布他林Terbutaline 80.4±6.8 15.4 99.0±7 12.4 73.8±5.8 7.1 齐帕特罗Zilpaterol 75.5±8.3 12.1 97.8±6.3 9.3 87.1±6.8 8.5 马布特罗Mabuterol 77.8±5.5 9.5 90.0±12.4 15.0 58.5±12.2 13.2 西马特罗Cimaterol 88.1±4.5 7.6 81.1±12.8 16.1 80.2±5.3 7.0 班布特罗Bambuterol 79.6±5.8 8.7 90.7±8.6 16.1 88.1±6.8 8.9 妥布特罗Tulobuterol 76.6±7.5 10.1 104.7±11.4 12.7 78.6±7.8 8.8 克仑普罗Clenproperol 96.8±4.3 12.2 83.7±32.4 10.6 76.6±8.7 9.2 福莫特罗Formoterol 94.7±4.2 11.9 81.0±15.6 13.1 77.2±8.3 13.1 西布特罗Cimbuterol 87.1±6.6 10.6 71.3±12.4 13.8 76.6±12.5 12.3 沙美特罗Salmeterol 78.9±7.8 11.6 112.1±7.2 11.8 70.7±8.8 14.0 利托君Ritodrine 82.3±5.5 14.4 102.4±7.1 13.3 78.6±12.6 8.4 克伦塞罗Clencyclohexerol 93.3±5.9 11.4 91.7±9.4 9.5 98.2±8.6 9.0