桃GRAS家族全基因组鉴定与响应UV-B的表达模式分析

李晨,赵雪惠,王庆杰,王旭旭,肖伟,陈修德,付喜玲,李玲,李冬梅

Genome Identification of PpGRAS Family and Expression Pattern Analysis of Responding to UV-B in Peach

Chen LI,XueHui ZHAO,QingJie WANG,XuXu WANG,Wei XIAO,XiuDe CHEN,XiLing Fu,Ling LI,DongMei LI

表1 48个PpGRAS的qRT-PCR引物序列

Table 1 Primer sequences of 48 PpGRAS genes used for qRT-PCR

引物名称 Locus name 上游序列（5'-3'） Forward primer (5'-3') 下游序列（5'-3'） Reverse primer (5'-3') Prupe.1G119100 CACCCTTCTTTGTCACACGG CCCAGCCCTCACATTCCTAA Prupe.1G119200 AACAATTGTGGGGACTTCGC ACCTGCCCTACAAACCCTAC Prupe.1G199300 TCCTCTCCGAGTTCAACCAC CGAGCCTTATCCCGGAATCT Prupe.1G237500 ACTGGAGGAGCTCTGAGAGA ATGACGTTCCTGATTTCGCG Prupe.1G329600 CTGAGTTCGCAATGAGGAGC GGGCCTCAATAAAACGGGTC Prupe.1G501500 GCATGCGTTCGATCTGGATT CAATGAGCTCGTGGAAGTCG Prupe.2G028700 CCATCCTCATCGGCTCAGAT CTTTCTCGGCGAGCTCTTTC Prupe.2G138400 CACCACCTGACCAGTACCAT TCCGTTTCTGGCAGGGTATT Prupe.2G237300 GAGGGAAAGGAAAGGGTGGA TCCTTCCATCCCAACAGCAT Prupe.2G299400 TTAGCTTTGAAGTCCCGGGT ATTCGTCGCTGATTTGTCCG Prupe.3G040400 GCGGATTAGTGCAGAGATGC GACTCCTGATAGCCTCCGTC Prupe.3G162500 TCAAGGCAGCCAGTGGATTA AATTGCCTCTCCTGGTCGAA Prupe.3G187000 TGGTGGACGAAGATGCAGAT GAACCCCTCGTACGCTATCA Prupe.3G201900 GCATGCGAAGGAGAGGAAAG TCCAAAATGAAGTGCACCGG Prupe.3G222800 CACCCTTCTTTGTCACACGG CCCAGCCCTCACATTCCTAA Prupe.3G249700 ACGACTCTGAGCCACAAGAA TTCTGCTTCTTTGAACCCGC Prupe.3G309800 GCAGCATTCTTCCCCGTATG GCCAAGAAATGTGACACCGT Prupe.4G007500 AGGGGTAGGGGAAGCTAGAA TGCTTCTCCCTTCTTCTGCA Prupe.4G132100 AGTGGTGGCTATTTCGGTGA GCCTCCTCAACTCCCTTCTT Prupe.4G200200 TCTTCCACTTCCAAGCGCTA CCCAGCCCTCACATACCTAG Prupe.4G233000 TGGCAGGCTAGGTATGTGAG CTTTCCATCCCTGCAGCATC Prupe.4G258200 TCTTCCACTTCTCTGCCCTG GCAAACCAGCCCTTGTACAA Prupe.5G072300 AGAGTTGTGAGGCCTGAGAC CTTCCATCCTTGCAGCATCC Prupe.5G091700 TCTGAGCACAAAGAGGGGAG GCCTTATCTGCCTGAGTTGC Prupe.5G156100 GCTGGCGATTGTTAACGGAT ATCCTTTCTGAACCCTCGCA Prupe.6G037000 CCCTCTCTTCTTCAACCGGT CAAAACCTGCCCTCCTCAAC Prupe.6G073300 TTGGTCAATCTTTGGAGCGC GAACCCCTTCTCCCATGTCA Prupe.6G073400 GGCCACAAGTTCCTCCTTTG CTACCGCCAAGTTGCTCATC Prupe.6G073500 GTTGACGGGCTTGACTTTGT ACTAAACGTCACGGGGCTAA Prupe.6G073600 TTTTGCAAGGCCACAGACTC ACCGTTTGAAGCCACATGAC Prupe.6G073700 GACATCGATGAGGAGGAGCT TGTGGATCAAATGGAGGCCT Prupe.6G073800 TGGTCATGTCCGAGGTGTAC CCATTGTTCTCCTCCACCCT Prupe.6G073900 CGATGAACCAAGGGATGCAG TCGCCACAAACCCTCTGTAT Prupe.6G074000 TCGAAATCTCCGAGCTCTCC ATTGGCGGTGAAGTGAGAGA Prupe.6G220800 GATTTGGCGACGCTGAGATT GCTCCACCATATGCCTCTCT Prupe.6G223200 AGGAAGGAGGAAGGGGAGAT TTGACTTGGTTATGGGGCCT Prupe.6G257000 AAAGATGGAGGACACTGGCA GGTTGTCTCATTGTGCTGCA Prupe.6G282800 CTGGCTATCTCCTGGGATGG TCAGGAGAGGCTAGCTGAGA Prupe.6G300900 GGAGGGTGTGGAGAGAGAAG AGAGAGGCCTTTCGTTCCAA Prupe.7G150900 CGCTTTCTCTCTCACTCCCT CAGAAAACCGGAACCCAGTG Prupe.7G153700 ACGAAGGGTCATCATCTGCA TGGGAAATTTGCTCAAGGGC Prupe.7G236100 ACTCACTCGATGCTAGCCTC CCAAAGTGTACCCATCGCAG Prupe.8G016200 CCCCTTCATCAAGTTTGCCC ATGTTCCCACTCCGGTCATT Prupe.8G054000 TGAGGGAAATGGAGCAGAGG GCCACACGCTGAAAAGAAGA Prupe.8G220900 TTGCATGCCACCAAGAAGTC TCTTCTGCTTGCCTGCTACT Prupe.8G266100 TCTCATGCACCTCCGATCTC ATATGGAATGGTCGGGCGAT Prupe.I004400 AAGACCAGTCACTTCTCCGG CGAAACGCTGCTTCCGATTA Prupe.I004500 CAAGAGCTATTGGTTCGGGC CCTGTTTCGATCAGCTCTGC Actin GTTATTCTTCATCGGCGTCTTCG CTTCACCATTCCAGTTCCATTGTC