鸭寡腺苷酸合成酶样蛋白原核表达及多克隆抗体制备
毕可然1,2,李银1(
),韩凯凯1,赵冬敏1,刘青涛1,刘宇卓1,黄欣梅1,杨婧1
),韩凯凯1,赵冬敏1,刘青涛1,刘宇卓1,黄欣梅1,杨婧1
Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Oligoadenylate Synthase-Like Protein
BI KeRan1,2,LI Yin1(),HAN KaiKai1,ZHAO DongMin1,LIU QingTao1,LIU YuZhuo1,HUANG XinMei1,YANG Jing1
),HAN KaiKai1,ZHAO DongMin1,LIU QingTao1,LIU YuZhuo1,HUANG XinMei1,YANG Jing1
图4. 融合蛋白SDS-PAGE和Western-blotting检测
M：蛋白marker;1：IPTG诱导前;2：IPTG诱导后全菌;3：IPTG诱导后沉淀;4：IPTG诱导后上清
Fig. 4. The result of SDS-PAGE and Western-blotting from recombinant protein
M: Protein marker; 1: Before IPTG induction; 2: All bacteria induced by IPTG; 3: Precipitation induced by IPTG; 4: Supernatant induced by IPTG
