表达H9亚型禽流感病毒HA基因重组鸭肠炎病毒的构建
孙莹,张兵,李岭,黄小洁,侯力丹,刘丹,李启红,李俊平,王乐元,李慧姣,杨承槐(
)
)
Construction of a Recombinant Duck Enteritis Virus Expressing Hemagglutinin of H9N2 Avian Influenza Virus
SUN Ying,ZHANG Bing,LI Ling,HUANG XiaoJie,HOU LiDan,LIU Dan,LI QiHong,LI JunPing,WANG LeYuan,LI HuiJiao,YANG ChengHuai()
)
图1. 重组病毒的构建示意图
a：DEV基因组示意图,包括UL, IRS, US和TRS区域;b：GFP基因表达盒插入到DEV gE基因内,获得rDEV-△gE-GFP-gpt;c：质粒pT-gE-HA与rDEV-△gE-GFP-gpt进行同源重组,获得重组病毒rDEV-△gE-HA
Fig. 1. Construction of recombinant DEV
a: Map of the DEV genome, which consists of long (UL) and short (US) unique regions with inverted repeat sequences (IRS,TRS) flanking the US region; b: Construction of rDEV-△gE-GFP-gpt. An expression cassette encoding GFP was inserted in DEV genome; c: Plasmid pT-gE-HA was used to generate rDEV-△gE-HA by recombination with rDEV-△gE-GFP-gpt
