表达H9亚型禽流感病毒HA基因重组鸭肠炎病毒的构建

孙莹,张兵,李岭,黄小洁,侯力丹,刘丹,李启红,李俊平,王乐元,李慧姣,杨承槐

Construction of a Recombinant Duck Enteritis Virus Expressing Hemagglutinin of H9N2 Avian Influenza Virus

Ying SUN,Bing ZHANG,Ling LI,XiaoJie HUANG,LiDan HOU,Dan LIU,QiHong LI,JunPing LI,LeYuan WANG,HuiJiao LI,ChengHuai YANG

表1 目的基因的扩增引物及鉴定引物

Table 1 Primers for amplification and identification of target gene

引物名称 Primer name 序列（5′-3′） Sequence (5′-3′) H9Nhe I-F CAGCTAGC CGCCACCATGGAGACAGTATCACTA ATAACTA H9BHI-R CGGGATCCTTATATACATATGTTGCATCTGC Re-JD-F TCAGGATGTAACGCTGGAG Re-JD-R GGCATCGCAGTCGGTTTT