图1. 重组病毒的构建示意图
a：DEV基因组示意图,包括UL, IRS, US和TRS区域;b：PCR扩增UL56基因的上下游片段UL56-u,UL56-d;c：DEVΔ3513-RFP的构建,将RFP基因表达盒插入到DEV UL56基因组;d: 应用融合PCR从DEV亲本毒中扩增UL56-ud。PCR产物与DEVΔ3513-RFP进行同源重组,获得重组病毒DEVΔ3513;e: 应用PCR从DEV亲本毒中扩增UL56u-3 513 bp-UL56d。PCR产物与DEVΔ3513-RFP进行同源重组,获得重组病毒DEVΔ3513(R)

Fig. 1. Construction of recombinant DEV
a : Map of the DEV genome, which consists of long (UL) and short (US) unique regions with inverted repeat sequences (IRS,TRS) flanking the US region; b : Regions upstream (UL56-u) and downstream (UL56-d) of UL56 were amplified by PCR; c: Construction of DEVΔ3513-RFP. An expression cassette encoding RFP was inserted in DEV genome; d: UL56-ud were amplified from wt DEV and assembled together by SOE PCR. The product was used to generate DEVΔ3513 by recombination with DEVΔ3513-RFP; e: The region of the DEV genome encompassing UL56u-3 513 bp-UL56d was amplified by PCR. The product was used to generate DEVΔ3513(R) by recombination with DEVΔ3513-RFP