柱状苹果Co基因的筛选与候选基因分析

白团辉,李莉,郑先波,王苗苗,宋尚伟,焦健,宋春晖

Screening and Expression Analysis of Co Candidate Genes in Columnar Apple

TuanHui BAI,Li LI,XianBo ZHENG,MiaoMiao WANG,ShangWei SONG,Jian JIAO,ChunHui SONG

表2 苹果Co定位区间的基因初步筛选

Table 2 Preliminary screening of genes in apple Co region

基因编号 Gene ID 预测功能 Putative function 基因表达量Gene expression 普通型Normal 柱状Columnar MD10G1183600 肽酶S24/S26A/S26B/S26C家族蛋白 Peptidase family protein 1040.4 1080.7 MD10G1183700 染色体(SMC)家族蛋白的结构维持 (SMC) family protein 777.0 1174.6 MD10G1183800 非编码RNA ncRNA - - MD10G1183900 非编码RNA ncRNA - - MD10G1184000 前mRNA剪接Prp 18-相互作用因子 Pre-mRNA splicing Prp18-interacting factor 949.5 1446.9 MD10G1184100 MYB结构域蛋白15 MYB domain protein 15 11.4 230.1 MD10G1184200 非编码RNA ncRNA - - MD10G1184300 未知功能蛋白 Protein of unknown function 331.7 335.6 MD10G1184400 未知功能蛋白 Protein of unknown function 270.1 240.6 MD10G1184500 肌动蛋白解聚因子6 Actin depolymerizing factor 6 1209.1 1275.9 MD10G1184600 磷酸盐转运蛋白1 Phosphate transporter 1 - - MD10G1184700 磷酸盐转运蛋白1 Phosphate transporter 1 472.8 519.2 MD10G1184800 乙烯响应因子1 Ethylene response factor 1 2.8 0.0 MD10G1184900 非编码RNA ncRNA - - MD10G1185000 整合酶型DNA结合超家族蛋白 Integrase-type DNA-binding superfamily protein 2.8 0.0 MD10G1185100 整合酶型DNA结合超家族蛋白 Integrase-type DNA-binding superfamily protein - - MD10G1185200 未知功能蛋白 Protein of unknown function 518.3 640.6 MD10G1185300 环/U盒超家族蛋白 RING/U-box superfamily protein - - MD10G1185400 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein 0.0 26.4 MD10G1185500 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein - - MD10G1185600 环/U盒超家族蛋白RING/U-box superfamily protein 0.9 3.2 MD10G1185700 四三肽重复序列（TPR）样超家族蛋白 Tetratricopeptide repeat (TPR)-like superfamily protein - - MD10G1185800 四三肽重复序列（TPR）样超家族蛋白 Tetratricopeptide repeat (TPR)-like superfamily protein - - MD10G1185900 自噬9 Autophagy 9 (APG9) 420.7 515.0 MD10G1186000 结节蛋白MTN21/EAMA样转运蛋白家族 Nodulin MTN21/EAMA-like transporter family protein 84.3 108.7 MD10G1186100 无顶端分生组织结构域转录调节超家族蛋白 NAC (No Apical Meristem) domain transcriptional regulator superfamily protein - - MD10G1186200 非编码RNA ncRNA - - MD10G1186300 开放阅读框7上游保守肽 Conserved peptide upstream open reading frame 7 966.5 1175.6 MD10G1186400 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein 41.7 11.6 MD10G1186500 糖基水解酶9B6 Glycosyl hydrolase 9B6 - - MD10G1186600 糖基水解酶9B1 Glycosyl hydrolase 9B1 - - MD10G1186700 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein - - MD10G1186800 碱性螺旋-环-螺旋（bHLH）DNA结合超家族蛋白 Basic helix-loop-helix (bHLH) DNA-binding superfamily protein - - MD10G1186900 碱性螺旋-环-螺旋（bHLH）DNA结合超家族蛋白 Basic helix-loop-helix (bHLH) DNA-binding superfamily protein - - MD10G1187000 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein 0.9 11.6 MD10G1187100 非编码RNA ncRNA - - MD10G1187200 α/β水解酶超家族蛋白Alpha/beta-Hydrolases superfamily protein 2.8 0.0 MD10G1187300 侧生器官边界结构域29 Lateral organ boundaries-domain 29 - - MD10G1187400 锌指Zinc finger - - MD10G1187500 光系统I亚单位H2 Photosystem I subunit H2 - - MD10G1187600 侧生器官边界结构域29 Lateral organ boundaries-domain 29 - - MD10G1187700 侧生器官边界结构域16 Lateral organ boundaries-domain 16 1.9 0.0 MD10G1187800 多聚ADP核糖聚合酶2 Poly(ADP-ribose) polymerase 2 869.9 577.3 MD10G1187900 ARM重复超家族蛋白 ARM repeat superfamily protein 560.0 506.6 MD10G1188000 BET1P/SFT1P样蛋白14A BET1P/SFT1P-like protein 14A 915.4 391.5 MD10G1188100 C2H2和C2HC锌指超家族蛋白C2H2 and C2HC zinc fingers superfamily protein 5.7 12.7 MD10G1188200 C2H2和C2HC锌指超家族蛋白C2H2 and C2HC zinc fingers superfamily protein - - MD10G1188300 核糖体S17家族蛋白 Ribosomal S17 family protein 0.0 2.1 MD10G1188400 具有Dil结构域的肌球蛋白家族蛋白 Myosin family protein with Dil domain 110.9 186.8 MD10G1188500 半乳糖氧化酶/kelch重复超家族蛋白 Galactose oxidase/kelch repeat superfamily protein - - MD10G1188600 未知Unknown - - MD10G1188700 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein - - MD10G1188800 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein - - MD10G1188900 未知Unknown 34.1 51.7 MD10G1189000 未知Unknown 292.8 93.9 MD10G1189100 未知Unknown 154.5 228.0 MD10G1189200 光合电子转移C Photosynthetic electron transfer C - - MD10G1189300 未知Unknown 145.0 162.5 MD10G1189400 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein - - MD10G1189500 未知Unknown 37.9 19.0 MD10G1189600 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein - - MD10G1189700 半乳糖氧化酶/kelch重复超家族蛋白 Galactose oxidase/kelch repeat superfamily protein - - MD10G1189800 未知Unknown - - MD10G1189900 2-氧戊二酸（2OG）和铁（II）依赖性加氧酶超家族蛋白 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein - - MD10G1190000 碱性螺旋-环-螺旋（bHLH）DNA结合超家族蛋白 Basic helix-loop-helix (bHLH) DNA-binding superfamily protein 668.0 853.8 MD10G1190100 未知Unknown 245.4 51.7 MD10G1190200 细胞色素P450 Cytochrome P450 531.6 331.4 MD10G1190300 未知Unknown - - MD10G1190400 细胞色素P450 Cytochrome P450 661.4 798.9 MD10G1190500 未知功能蛋白 Protein of unknown function 69.2 180.5 MD10G1190600 非编码RNA ncRNA 6.6 5.3 MD10G1190700 IND1（NADH脱氢酶所需的铁硫蛋白）-类 IND1(iron-sulfur protein required for NADH dehydrogenase)-like 225.5 520.3 MD10G1190800 类pfkb碳水化合物激酶家族蛋白 Pfkb-like carbohydrate kinase family protein 193.3 224.8 MD10G1190900 Mudr家族转座酶 Mudr family transposase 389.5 707.1 MD10G1191000 非编码RNA ncRNA 1.9 3.2 MD10G1191100 含核苷三磷酸水解酶超家族蛋白的P环 P-loop containing nucleoside triphosphate hydrolases superfamily protein 2.8 9.5 MD10G1191200 翼螺旋DNA结合转录因子家族蛋白 Winged-helix DNA-binding transcription factor family protein 10231.9 5165.8 MD10G1191300 乙烯响应元件结合因子3 Ethylene responsive element binding factor 3 1089.7 547.7 MD10G1191400 WRKY DNA结合蛋白21 WRKY DNA-binding protein 21 839.5 546.7 MD10G1191500 信号识别粒子 Signal recognition particle 508.8 376.8 MD10G1191600 跨膜氨基酸转运体家族蛋白 Transmembrane amino acid transporter family protein — — MD10G1191700 PRA1家族蛋白 PRA1 (Prenylated rab acceptor) family protein 321.2 246.9 MD10G1191800 DREB2A相互作用蛋白2 DREB2A-interacting protein 2 338.3 338.8 MD10G1191900 镁转运蛋白9 Magnesium transporter 9 364.8 421.1 MD10G1192000 半乳糖醛糖基转移酶6 Galacturonosyltransferase 6 188.6 193.1 MD10G1192100 光系统II反应中心W Photosystem II reaction center W 6877.5 1885.9 MD10G1192200 非编RNA ncRNA 7.6 2.1 MD10G1192300 谷胱甘肽还原酶 Glutathione reductase 665.2 1506.0 MD10G1192400 Transferin/WD 40重复超家族蛋白 Transducin/WD40 repeat-like superfamily protein 11.4 58.0 MD10G1192500 α/β水解酶超家族蛋白 Alpha/beta-Hydrolases superfamily protein 303.2 237.4 MD10G1192600 非编码RNA ncRNA MD10G1192700 非编码RNA ncRNA - - MD10G1192800 类RNI超家族蛋白 RNI-like superfamily protein 1226.2 1437.4 MD10G1192900 吲哚-3-乙酸7 Indole-3-acetic acid 7 3281.4 8731.8 MD10G1193000 AUX/IAA转录调节家族蛋白 AUX/IAA transcriptional regulator family protein - - MD10G1193100 原体prib/单链DNA结合 Primosome prib/single-strand DNA-binding 302.3 378.9 MD10G1193200 碱性螺旋-环-螺旋（bHLH）DNA结合超家族蛋白 Basic helix-loop-helix (bHLH) DNA-binding superfamily protein 525.0 624.8 MD10G1193300 色氨酸合酶α链 Tryptophan synthase alpha chain 329.8 215.3 MD10G1193400 碱性亮氨酸拉链25 Basic leucine zipper 25 145.9 575.2 MD10G1193500 GNAT家族1组蛋白乙酰转移酶 Histone acetyltransferase of the GNAT family 1 547.7 491.8 MD10G1193600 非编码RNA ncRNA - - MD10G1193700 泛素结合酶11 Ubiquitin-conjugating enzyme 11 - - MD10G1193800 SOUL血红素结合家族蛋白 SOUL heme-binding family protein 1072.7 1758.2 MD10G1193900 泛素特异性蛋白酶15 Ubiquitin-specific protease 15 810.2 1333.9 MD10G1194000 阳离子氨基酸转运蛋白8 Cationic amino acid transporter 8 532.5 255.4 MD10G1194100 拟南芥赤霉素2-氧化酶1 Arabidopsis thaliana gibberellin 2-oxidase 1 1936.8 795.7