苹果LIM基因家族生物信息学及表达分析

袁高鹏,韩晓蕾,卞书迅,张利义,田义,张彩霞,丛佩华

Bioinformatics and Expression Analysis of the LIM Gene Family in Apple

GaoPeng YUAN,XiaoLei HAN,ShuXun BIAN,LiYi ZHANG,Yi TIAN,CaiXia ZHANG,PeiHua CONG

表1 荧光定量PCR引物序列

Table 1 Primers of fluorescent quantitative PCR

基因 Gene 上游引物Forward primer (5′-3′) 下游引物Reverse primer (5′-3′) MdLIM1 ATGCAGCGCCTGTGATAAGA GCTCGAAATGAGGTTTGCAGT MdLIM2 GCTACTTGTGGTAAAACCGCT CCAAAGCCGCGTAGTTTGAG MdLIM3 ATGCCAAGTCAGTCTCAAACT TGGTATGGAGTCCCGTTG MdLIM4 GGGACCGTAGACAAATGTGC AGTTGGGCAAAGTGGTGCTT MdLIM5 AAAACTGTTCGAGAAAGATCCG GAAGAGTTGGCTATGGTGATGC MdLIM6 GACAGTCTATCCGCTGGAGA ACGATGAATGTGTAAGGGCA MdLIM7 GATGGGATTTCCTACCACAA CAGTCTCCTTGAACAGTTGCT MdLIM8 TCTCCTTTCTCAGGGACACC GCACTTATCTCTGGTTCCACC MdLIM9 TGCCTACCATAAGACCTGCT TCTCCTTGAAGAGTTGCTCG MdLIM10 ACTAGTTCCAGTACACAGACCAAC CTCCATGAGTGCACCGGAAA MdLIM11 TCTACCACAAAGCCTGCTTCC CAAGACTGCCGGTTCTTTTGA MdActin TGACCGAATGAGCAAGGAAATTACT TACTCAGCTTTGGCAATCCACATC