利用TRV-HIGS技术鉴定核盘菌致病相关的分泌蛋白基因
远俊虎,丁一娟,杨文静,闫宝琴,柴亚茹,梅家琴,钱伟(
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Identification of Genes Encoding Secretory Proteins Related to the Pathogenicity of Sclerotinia sclerotiorum Using TRV-HIGS
YUAN JunHu,DING YiJuan,YANG WenJing,YAN BaoQin,CHAI YaRu,MEI JiaQin,QIAN Wei()
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图3. TRV-HIGS转化烟草植株的鉴定
A：注射含有TRV-Gene重组质粒9 d后的烟草植株的PCR鉴定The identification of N. benthamiana at 9 days post TRV-Gene inoculation. M: Marker; 1: SS1G_00263; 2: SS1G_04945; 3: SS1G_03181; 4: SS1G_04343; 5: SS1G_03146; 6: SS1G_02250; 7: SS1G_11912; 8: SS1G_07655;B：注射不含重组质粒的重悬液9 d后的烟草植株的GFP信号观察GFP signal observation of N. benthamiana at 9 days after suspension without recombinant vectors inoculation;C：注射TRV-GFP载体9 d后的烟草植株的GFP信号观察GFP signal observation of N. benthamiana at 9 days post TRV-GFP inoculation。标尺Bar = 30 μm
Fig. 3. Identification of TRV-HIGS N. benthamiana
