利用TRV-HIGS技术鉴定核盘菌致病相关的分泌蛋白基因
远俊虎,丁一娟,杨文静,闫宝琴,柴亚茹,梅家琴,钱伟()
Identification of Genes Encoding Secretory Proteins Related to the Pathogenicity of Sclerotinia sclerotiorum Using TRV-HIGS
YUAN JunHu,DING YiJuan,YANG WenJing,YAN BaoQin,CHAI YaRu,MEI JiaQin,QIAN Wei()

图1. 候选基因在侵染抗、感病甘蓝过程中的表达热图
核盘菌候选基因在接种抗、感甘蓝茎秆(A)0、12、24 h和叶片(B) 0、6、12 h后的表达量Relative expression levels of candidate genes in the susceptible and resistant B. oleracea after inoculation with S. sclerotiorum in stem (A) post 0, 12, 24 h and leaf (B) post 0, 6, 12 h。Rs:抗病甘蓝材料的茎秆Resistant B. oleracea stem;Ss:感病甘蓝材料的茎秆Susceptible B. oleracea stem;Rl:抗病甘蓝材料的叶片Resistant B. oleracea leaf;Sl:感病甘蓝材料的叶片Susceptible B. oleracea leaf。以lg (FPKM+1)值绘制基因表达量的热图,红色表示高表达基因,蓝色表示低表达基因。颜色从红到蓝,表示lg (FPKM+1)从大到小。基因表达热图构建方法见文献[11] The lg (FPKM+1) value was used for the heatmap. Red indicated high expression genes and blue indicated low expression genes. The colors ranged from red to blue, indicating that lg (FPKM+1) from large to small. The heatmap was conducted according to reference [11]

Fig. 1. Heatmap of candidate genes of S. sclerotiorum in the process of infecting the resistant and susceptible B. oleracea