利用TRV-HIGS技术鉴定核盘菌致病相关的分泌蛋白基因

远俊虎,丁一娟,杨文静,闫宝琴,柴亚茹,梅家琴,钱伟

Identification of Genes Encoding Secretory Proteins Related to the Pathogenicity of Sclerotinia sclerotiorum Using TRV-HIGS

JunHu YUAN,YiJuan DING,WenJing YANG,BaoQin YAN,YaRu CHAI,JiaQin MEI,Wei QIAN

表2 候选基因的结构域功能分析

Table 2 Putative functions of the domains in candidate genes

基因 Gene 结构域名称 Domain name 结构域位置 Location (aa) E期望值 E-value 可能的功能或特性 Putative function or feature SS1G_00263 SCOP:d1hw1a2 14-79 0.77 调节药物的耐受性 Regulation of drug tolerance SS1G_04945 Pfam:Glyco_hydro_7 21-446 2.6e-200 O型糖苷水解酶 O-glycoside hydrolase PDB:3PL3|A 19-446 0 纤维二糖水解酶 Cellobiohydrolase SCOP:d1gpia_ 19-450 0 伴刀豆球蛋白A样凝集素/葡聚糖酶 Concanavalin A/Glucanase SS1G_03181 Pfam:ASP 91-399 3.2e-69 天冬氨酰蛋白酶（酸性蛋白酶）Aspartyl proteases (acid proteases) SCOP:d1bxoa_ 79-399 1e-81 酸性蛋白酶 Acid proteases PDB:3APP|A 83-398 1e-90 酸性蛋白酶 Acid proteases SS1G_04343 SCOP:d1gh8a_ 339-377 0.39 翻译延长因子 Translation elongation factor SCOP:d1fuia2 411-457 0.68 异构酶 Isomerase SS1G_03146 SCOP:d1h4vb2 18-62 1.8 生物素合成酶 Biotin synthetase SS1G_02250 SCOP:d1ed8a_ 43-90 5.7 碱性磷酸酶 Alkaline phosphatase SCOP:d1dofa_ 215-241 0.86 L型天冬氨酸 L-aspartic acid SS1G_11912 Pfam:NPP1 52-242 4.5e-61 坏死诱导蛋白 Necrosis inducing protein PDB:3GNZ|P 32-242 4e-65 毒素 Toxin SCOP:d1fuia2 188-240 0.58 异构酶 Isomerase SS1G_07655 Pro-kuma_activ 35-181 3.25e-19 肽酶 Peptidase PDB:3EDY|A 35-581 2e-67 肽酶 Peptidase SCOP:d1gt91_ 36-581 9e-19 枯草杆菌蛋白酶类 Subtilisins