利用TRV-HIGS技术鉴定核盘菌致病相关的分泌蛋白基因

远俊虎,丁一娟,杨文静,闫宝琴,柴亚茹,梅家琴,钱伟

Identification of Genes Encoding Secretory Proteins Related to the Pathogenicity of Sclerotinia sclerotiorum Using TRV-HIGS

JunHu YUAN,YiJuan DING,WenJing YANG,BaoQin YAN,YaRu CHAI,JiaQin MEI,Wei QIAN

表1 本试验所用引物信息

Table 1 Primers used in this study

基因 Gene 引物序列 Primer sequence (5′-3′) 产物大小 Product size (bp) 用途 Usage SS1G_00263 F: CCGGAATTCCGGCAGCGCCTCAAGCTCGACTCAAATC R: CGAGCTCGCTGACGGTAGCGGAACCAACAACGG qF: TCTTTGAGGATGGAACTTGGAC qR: AGCCTGGCAAGCATAATCG 273 基因扩增Gene amplification qRT-PCR SS1G_04945 F: CCGGAATTCCGGTCTCCTTCCTTCTCGGC R: CGAGCTCGCTCGTAATCGGCACCAT qF: TCTCAACGGTGCTCTTTACTTC qR: TTGCTATCAGGGACCCATCC 263 基因扩增Gene amplification qRT-PCR SS1G_03181 F: CCGGAATTCCGGTGAGCGATGCTACCAACAGCGCCTAC R: CGAGCTCGCACCAGCACTTTGGAGAGCACCGTAA qF: TGCCGATTACGAGGGAACA qR: TGGAAACATCGACGGTGAAG 210 基因扩增Gene amplification qRT-PCR SS1G_04343 F: CCGGAATTCCGGTGGTCTTTACGCTGGGTATTTC R: CGAGCTCGCACCGTTGGTTGTGTTTTCATT qF: TGGTCTTTACGCTGGGTATTTC qR: CAGACACTTGCGAATGGAGC 284 基因扩增Gene amplification qRT-PCR SS1G_03146 F: CCGGAATTCCGGACATTTCCTCTTGAACCATCCCGTA R: CGAGCTCGTTTATGACACCCTTGTTTCCAGCGA qF: GCACATTTCCTCTTGAACCATC qR: GCAGTGTCACTTCCCACCATT 309 基因扩增Gene amplification qRT-PCR SS1G_02250 F: CCGGAATTCCGGTCACACTTTTGGCATT R: CGAGCTCGATCAGCACGTTTTTCT qF: AAGCCAACACCAACCTCATC qR: CACTGGAGCGTAGTTCTCGTAG 272 基因扩增Gene amplification qRT-PCR SS1G_11912 F: CCGGAATTCCGGTCAGCAGCTCCACCTCCACCACC R: CGAGCTCGATTCGCCTTTCCAAAATCCGTAT qF: TTCCCGAAACCGTTCCTAGT qR: TCACCATTGCTACTGCCACTT 257 基因扩增Gene amplification qRT-PCR SS1G_07655 Sstub1 （内参基因Actin gene） F: CCGGAATTCCGGGCTACTGTTCCTTTGGACTACGCT R: CGAGCTCGTTACTGAGGAGTGAGTCGTGTCGG qF: AATATGCCAGAGCCATCACA qR: CAGCGTAGTCCAAAGGAACAG qF: GTGAGGCTGAGGGCTGTGTGA qR: CCTTTGGCGATGGGACG 322 基因扩增Gene amplification qRT-PCR qRT-PCR