黑龙江省稻瘟病菌无毒基因AVR-Pib、AVR-Pik和AvrPiz-t的检测与分析

孟峰,张亚玲,靳学慧,张晓玉,姜军

Detection and Analysis of Magnaporthe oryzae Avirulence Genes AVR-Pib, AVR-Pik and AvrPiz-t in Heilongjiang Province

Feng MENG,YaLing ZHANG,XueHui JIN,XiaoYu ZHANG,Jun JIANG

表1 用于扩增稻瘟病菌无毒基因的引物

Table 1 The primers for M. oryzea avirulence genes amplification

无毒基因 Avirulence gene 引物序列 Primer sequence (5′-3′) 目的片段长度 Length of targeted fragments (bp) 目的 Purpose AVR-Pib F1: AAGTCCTTCCCATTACCCTA R1: GCAATAACCATCCAGCCATA 484 检测AVR-Pib CDS Detection of AVR-Pib CDS F2: GAAGTACCCACCCATAACCC R2: CAAGGGAGGCAAATCTAACC 1147 检测AVR-Pib基因全长 Detection of AVR-Pib gene full length AVR-Pik F1: AATTTATTCAACTGCCACTCTG R1: AACCTCGTCAAACCTCCCTA 526 检测AVR-Pik CDS Detection of AVR-Pik CDS F2: GACAAACAGGATGGGATT R2: AGGTCGTAGGTCGGAAAC 1929 检测AVR-Pik基因全长 Detection of AVR-Pik gene full length AvrPiz-t F1: ATCAAATGAACACCAGGAA R1: CCAGCCGAAGATACAAAA 450 检测AvrPiz-t CDS Detection of AvrPiz-t CDS F2: AATCTCCCAATGGTTCGC R2: AAAGTGGCTCGTTCCTAA 1965 检测AvrPiz-t基因全长 Detection of AvrPiz-t gene full length