磷胁迫下羊草的响应及磷响应相关基因表达分析

万东莉,侯向阳,丁勇,任卫波,王凯,李西良,万永青

Response and the Expression of Pi-Responsive Genes in Leymus chinensis Under Inorganic Phosphate Treatment

DongLi WAN,XiangYang HOU,Yong DING,WeiBo REN,Kai WANG,XiLiang LI,YongQing WAN

表1 基因和qRT-PCR引物特性

Table 1 Genes and characteristics of qRT-PCR primers

基因名称 Gene name 基因描述 Gene description 引物序列 Primer sequences (5′-3′) 引物扩增效率 Amplification efficiency (%) 产物大小 Amplicon length (bp) 溶解温度 Product melting temperature (℃) LcTUA α-微管蛋白Alpha-tubulin F: ACGAGGCCATCTACGACATCT 95.0 135 87.6 R: CCACGTTCAGGGCACCAT LcTUB β-微管蛋白Beta-tubulin F: TCCCTCGCCTCCACTTCTT 107.2 197 88.8 R: CTCCTTTGTGCTCATCTTCCC LcGAPDH 甘油醛3-磷酸脱氢酶Glyceraldehyde-3-phosphate dehydrogenase F: AAACGCAACGAAGCAACTACA 97.7 105 82.2 R: AGAGCACCAAGCCGCAAG LcEF1α 延伸因子1-α Elongation factor 1-alpha F: CCGCCATCAAGAAGAAATAGG 103.9 98 82.6 R: CGCCGAAGCAGGAATAAAA Lc18SrRNA 18S核糖体RNA 18S ribosomal RNA F: TTGAAGCTGATAGGAGGGAGG 96.7 98 82.8 R: CTGAAGGTTGGGCGGAATA LcCAP 腺苷酰环化酶相关蛋白 Adenylyl cyclase-associated protein F: AGGACGGGCAGTTCACCA 103.6 114 83.1 R: ACAAGGCAAAGGCAGCATC LcAPRT 腺嘌呤磷酸核糖转化酶 Adenine phosphoribosyl transferase F: GAATCCTCTGGCTTCAACACC 101.4 118 83.7 R: GGACATCACAACCTTGCTTCTT LcACT 肌动蛋白Actin F: ATTGTGCTCAGTGGTGGGTCA 106.5 137 85.5 R: CCAATCCAAACACTGTACTTCCTC LcPHO1-2 磷酸盐1-2 Phosphate1-2 F: CTCGCCTACTGGATTTCTCCC - 97 82.5 R: CCAACATTGTTCAAGTGTTCGTTC LcPAP2 紫色酸性磷酸酶2 Purple acid phosphatase2 F: CTCGCGTCCGCACACATAAT - 83 82.9 R: TCCGCTTCCAGCCACTTATA LcPAP27 紫色酸性磷酸酶27 Purple acid phosphatase27 F: CGACTCCTTCACCATCCACA - 172 88.3 R: TTCACGCACACGAGATTCTTT