乙烯利和1-MCP对菠萝蜜果实中AheAAT和 AheERF表达的影响

任雪岩,刘光财,李国鹏,叶春海,丰锋,王俊宁

Effects of Ethephon and 1-MCP on the Expression of AheAAT Gene and AheERF Transcription Factors in Jackfruit Fruit

XueYan REN,GuangCai LIU,GuoPeng LI,ChunHai YE,Feng FENG,JunNing WANG

表2 定量PCR引物序列

Table 2 Quantitative PCR primer sequence

引物名称 Primers name 引物序列 Primer sequence AheAAT-qup 5′- CATCAACGCCATCCTCAT -3′ AheAAT-qdw 5′-TGAAACCTAAGCCCTCCT -3′ AheERF1-qup 5′-CCCGCACCAACTTCCCTT -3′ AheERF1-qdw 5′-GTTGGGTTTCGTGAGAATGCC -3′ AheERF2-qup 5′-CGAACCAGGCGATAAAGGA -3′ AheERF2-qdw 5′-TGCCGAGCCACACCCTTA -3′ AheGAPDH-qup 5′-TTGAAGGGTGGNGCNAARAARG -3′ AheGAPDH-qdw 5′-ATAACCCCAYTCRTTRTCRTAC -3′