陆地棉扩展蛋白基因的鉴定与特征分析

张奇艳,雷忠萍,宋银,海江波,贺道华

Identification and Characterization of the Expansin Gene Family in Upland Cotton (Gossypium hirsutum)

QiYan ZHANG,ZhongPing LEI,Yin SONG,JiangBo HAI,DaoHua HE

表1 实时荧光定量PCR引物

Table 1 A list of primers used for qRT-PCR analyses

基因名称Gene name 正向引物Forward primers (5′-3′) 反向引物Reverse primers (5′-3′) GhEXLA1A TCACTGACATTGCAAAAGAAGG GAACACCATTGATTGTGTAGCA GhEXLA3A GTGATTACAGCCATTGGTGATG CCAAAACAAAAGCTCTCAAAGACTC GhEXLA2D TAACATGCAAAGAAAGTGAGGC AATCCATGGTTTTCCGGAAATG GhEXLA3D CTTTCGGGTCACTCAAAAATGT AATGCTTCTCAACATCCCAAAC GhEXPA06A CTTTCGGGTCACTCAAAAATGT AATGCTTCTCAACATCCCAAAC GhEXPA16A GAATCGTCCCCATTTCATTCAG CACCACCAACATTTGTAGTGAG GhEXPA25A CACCATCATTGTAACTGCTACG ATTTTGAACTTAACACCACCGG GhEXPA06D GCACTGCTTTGTTTAACAATGG ATTAGGGAGAGCATTGTTTGGA GhEXPA17D TTGTTGGGTTTTCTAGCAATGG CTGCAGTATTTGTTCCATAGCC GhEXPA19D GGAAACCTGTACAGTCAAGGTA GTTATGGTTCGACTAATGCACC GhEXPA20D CCTAACTTAGCATTGTCCAACG TTTCTTCATGCAGGGTACTCTT GhEXPA23D CGGCCACTCATACTTTAACTTG CTTCGTATGTTTGTCCGAACTG GhEXPA24D GAACGAAGAAAACACCCATCTT CTCTTCAGCCAAAGCATCTTAC UBQ7 GAAGGCATTCCACCTGACCAAC CTTGACCTTCTTCTTCTTGTGCTTG