沙葱萤叶甲气味结合蛋白GdauOBP20的基因克隆、 原核表达及其结合特性
李玲,谭瑶,周晓榕,庞保平(
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Molecular Cloning, Prokaryotic Expression and Binding Characterization of Odorant Binding Protein GdauOBP20 in Galeruca daurica
Ling LI,Yao TAN,XiaoRong ZHOU,BaoPing PANG()
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图3. GdauOBP20 重组蛋白的诱导表达（A）和分离纯化（B）
M：蛋白分子量标准 Protein molecular weight marker;1：未诱导对照组 Control group without induction;2—6：经不同浓度IPTG诱导的表达产物（0.1、0.5、0.8、1.0、1.5 mmol·L-1） Expressed products induced by different concentrations of IPTG;7：上清蛋白 Protein in bacterial supernatant;8：包涵体蛋白 Protein in bacterial inclusion;9：纯化的GdauOBP20重组蛋白 Separated and purified recombinant protein GdauOBP20
Fig. 3. Expression induced by IPTG (A) and purification (B) of recombinant protein GdauOBP20
