沙葱萤叶甲气味结合蛋白GdauOBP20的基因克隆、 原核表达及其结合特性

李玲,谭瑶,周晓榕,庞保平

Molecular Cloning, Prokaryotic Expression and Binding Characterization of Odorant Binding Protein GdauOBP20 in Galeruca daurica

Ling LI,Yao TAN,XiaoRong ZHOU,BaoPing PANG

表2 GdauOBP20重组蛋白与所测配基的亲和力

Table 2 Binding affinities of selected ligands with recombinant GdauOBP20

化学配基 Chemical ligand IC50 (μmol·L-1) 解离常数Ki Dissociation constant (μmol·L-1) 二烯丙基硫醚 Diallyl sulfide 42.30 42.10 二烯丙基二硫 Diallyl disulphide 50.97 50.73 二烯丙基三硫醚 Diallyl trisulfide — — 二甲基二硫醚 Dimethyl disulfide 31.79 31.64 二甲基三硫醚 Dimethyl trisulfide 31.16 31.01 1,3-二噻烷 1,3-Dithiane 49.86 49.63 己醛Hexanal 49.05 48.83 2-己烯醛2-Hexenal 37.97 37.80 苯甲酸甲酯 Methyl benzoate 37.37 37.19 对二甲苯p-Xylene 23.07 22.91 顺-2-己烯-1-醇 (Z)-2-Hexen-1-ol 47.01 46.80 月桂烯Myrcene 116.83 116.29 环庚三烯 1,3,5-Cycloheptatriene 26.68 26.55