沙葱萤叶甲气味结合蛋白GdauOBP20的基因克隆、 原核表达及其结合特性

李玲,谭瑶,周晓榕,庞保平

Molecular Cloning, Prokaryotic Expression and Binding Characterization of Odorant Binding Protein GdauOBP20 in Galeruca daurica

Ling LI,Yao TAN,XiaoRong ZHOU,BaoPing PANG

表1 本研究所用引物

Table 1 Primers used in this study

名称 Name 引物序列Primer sequence (5′-3′) 引物用途 Use of primers GdauOBP20-5′ GSP CACGGTCGAAAATTCCTTTTTTGCC RACE末端扩增 RACE end amplification GdauOBP20-3′ GSP TTGGCCCCATCTAAAGTTGCCGACG GdauOBP20-5′ NGSP TGGATCCGTAATCTCCATTAAGGGA 巢氏PCR Nested PCR GdauOBP20-3′ NGSP TGGAGCTTGTCACAACAAAGTCGCA GdauOBP20-F1 ATGTTTCGGGAGCTTCTA 中间片段扩增 Middle fragment amplification GdauOBP20-R1 TTAGAATATTATAAAGAGTTC GdauOBP20-F2 AGTCACACACCATGTTTCG cDNA全长验证 Verification of full-length cDNA GdauOBP20-R2 GATTTTTATTTATTTCTACAAAGAG GdauOBP20-F3 GAATTCAATCCCTTAATGGAGAT 原核表达 Prokaryotic expression GdauOBP20-R3 CTCGAGTTAGAATATTATAAAGAGTT