图6. 重组蛋白UGT73C19酶活产物的HPLC图
上图为UGT73C19重组蛋白的酶活产物,下图为失活蛋白的对照产物;a：山奈酚;b：槲皮素;c：杨梅素;d：芹黄素;e：大豆苷元;f：染料木素。K：山奈酚;A：芹黄素;M：杨梅素;D：大豆苷元;Q：槲皮素;G：染料木素;K5G：3-O-葡萄糖苷山奈酚;A5G：5-O-葡萄糖苷芹黄素;Q5G：5-O-葡萄糖苷槲皮素;Q4G：4-O-葡萄糖苷槲皮素;Q7G：7-O-葡萄糖苷槲皮素;D7G：7-O-葡萄糖苷大豆苷元;M7G：7-O-葡萄糖苷杨梅素;G7G：7-O-葡萄糖苷染料木素;通过保留时间、紫外吸收谱、质谱结构和文献综合比对分析

Fig. 6. Analysis of the products from enzymatic activity reaction of the recombinant UGT73C19 protein
Representative HPLC chromatograms of reaction products of UGT73C19 with Kaempferol (a); Quercetin(b); Myricetin(c); Apigenin(d); Daidzein (e) and Genistein (f). K: Kaempferol; A: Apigenin; M: Myricetin; D: Daidzein; Q: Quercetin; G: Genistein; K5G: kaempferol 3-O-glucoside; A5G: apigenin 5-O-glucoside; Q5G: quercetin 5-O-glucoside; Q4G: quercetin 4-O-glucoside; Q7G: quercetin 7-O-glucoside; D7G: daidzein 7-O-glucoside; M7G: myricetin 7-O-glucoside; G7G: genistein 7-O-glucoside; they were identified with retention time, UV spectrum, mass spectrum, and references