大豆类黄酮糖基转移酶基因UGT73C19的功能研究
狄少康,尹青岗,夏亚迎,庞永珍

Functional Characterization of a UDP: Flavonoid Glycosyltransferase Gene UGT73C19 in Glycine max
ShaoKang DI,QingGang YIN,YaYing XIA,YongZhen PANG
表1 重组UGT73C19蛋白的酶动力学分析
Table 1 Analysis of the enzymatic kinetics of the recombinant UGT73C19 protein
底物 Substrates Vmax(nmol·min-1 Km(μmol·L-1 Kcat(s-1 Kcat/Km(s-1?M-1
Kaempferol 0.113±0.026 186.400±113.700 0.051±0.008 297.622±92.697
Quercetin 0.269±0.116 403.730±189.080 0.120±0.052 302.504±16.544
Myricetin 0.022±0.009 34.690±14.520 0.010±0.004 291.258±9.472
Apigenin 0.014±0.001 76.870±4.430 0.008±0.000 101.608±0.789
Daidzein 0.011±0.001 85.160±6.400 0.006±0.000 70.010±2.522
Genistein 0.008±0.000 42.470±0.490 0.004±0.000 97.918±2.819