家蚕热休克蛋白HSP60相互作用蛋白筛选与鉴定

董战旗,蒋亚明,潘敏慧

Screening and Identification of Candidate Proteins Interacting with BmHSP60 in the Silkworm (Bombyx mori)

DONG ZhanQi,JIANG YaMing,PAN MinHui

图3 免疫荧光验证BmHSP60和BmHSP90、BmANT1相互作用 在BmN-SWU1细胞中转染BmHSP60和BmHSP90、BmANT1 48 h后,用Alexa 555标记抗Flag,FITC标记抗HA和Hoechst33258染色。红色荧光表示Flag,绿色荧光表示HA,蓝色荧光表示细胞核。比例尺Scale bar：5 μm

Fig. 3 Immunofluorescence verification of interaction between BmHSP60 with BmHSP90 and BmANT1 Co-localization of BmHSP60 with BmHSP90 and BmANT1 in BmN-SWU1 cells. Alexa 555 labels anti-Flag, FITC labels anti-HA and Hoechst33258 at 48 h post-transfection in the BmN-SWU1 cells. Red fluorescence represents Flag, green fluorescence represents HA, and blue fluorescence represents the nucleus