家蚕热休克蛋白HSP60相互作用蛋白筛选与鉴定

董战旗,蒋亚明,潘敏慧

Screening and Identification of Candidate Proteins Interacting with BmHSP60 in the Silkworm (Bombyx mori)

DONG ZhanQi,JIANG YaMing,PAN MinHui

图2 免疫共沉淀验证BmHsp90、BmANT1与BmHsp60相互作用 A：通过Western blot分析BmHSP60相互作用蛋白表达The expression of candidate interaction proteins of BmHSP60 by Western blot;B—E：通过Western blot分析BmHSP60相互作用蛋白。BmN-SWU1细胞共同转染BmHSP60和BmTubulin、BmEF-1α、BmHSP90、BmANT1。转染96 h后,收集细胞用Flag抗体共沉淀蛋白,靶蛋白用HA抗体检测。每个相互作用顶部表示免疫沉淀所用抗体,右侧标记显示Western blot分析所用抗体

Fig. 2 Western blot analysis determining the interaction between BmHsp60 with BmHsp90 and BmANT1 Co-immunoprecipitation of BmHSP60 examined by Western blot. BmN-SWU1 cells are co-transfected with BmHSP60 and candidate BmTubulin, BmEF-1α, BmHSP90, BmANT1 protein. At 96 hours after transfection, cells are lysed and immunoprecipitation performed with α-FLAG, and use α-HA to detect the bound of target protein. The label on the top of each panel shows the antibodies used for immunoprecipitation. The label on the right of each panel shows the antibodies used for analysis of Western blot