家蚕热休克蛋白HSP60相互作用蛋白筛选与鉴定

董战旗,蒋亚明,潘敏慧

Screening and Identification of Candidate Proteins Interacting with BmHSP60 in the Silkworm (Bombyx mori)

DONG ZhanQi,JIANG YaMing,PAN MinHui

表1 本试验所用引物

Table 1 Primers used in this study

引物名称Primer name 引物系列Primer sequence (5′-3′) BmHSP60 Flag-BamH I CGCGGATCCATGGACTACAAAGACGATGACGACAAGTTGCGTCTACCTCGTGTT BmHSP60Flag-Not I ATAAGAATGCGGCCGCTCACTTGTCGTCATCGTCTTTGTAGTC CATCATGCCTCCCATACCA BmANT1HA-KpnI-F GGGGTACCATGTACCCATACGACGTCCCAGACTACGCTTCGAACCTCGCCGA BmANT1 HA-SpeI-R GGACTAGTGGCGTAGTCGGGCACGTCGTAGGGGTACAGGACCTTCTTGATCTCAT BmTubulin HA-KpnI-F GGGGTACCTACCCATACGACGTCCCAGACTACGCTATGAGGGAATGCATCTCC BmTubulin HA-SpeI-R GGACTAGTGGCGTAGTCGGGCACGTCGTAGGGGTAATATTCATTTTCTTCGTCCAACT BmEF-1αHA-KpnI-F GGGGTACCTACCCATACGACGTCCCAGACTACGCTATGGGTAAAGAGAAGACTCATATCA BmEF-1αHA-SpeI-R GGACTAGTGGCGTAGTCGGGCACGTCGTAGGGGTATTTTTTCTTCTGGGCCTTCT BmHSP90 HA-SpeI-F GGACTAGTTACCCATACGACGTCCCAGACTACGCTATGAAGGTTGAAACCTTCAC BmHSP90 HA-XhoI-R CCGCTCGAGGGCGTAGTCGGGCACGTCGTAGGGGTACTGAATCGGCTCATCTT