侵染我国主要蔬菜作物的病毒种类、分布与发生趋势

刘勇,李凡,李月月,张松柏,高希武,谢艳,燕飞,张安盛,戴良英,程兆榜,丁铭,牛颜冰,王升吉,车海彦,江彤,史晓斌,何自福,吴云锋,张德咏,青玲,严婉荣,杨学辉,汤亚飞,郑红英,唐前君,章松柏,章东方,蔡丽,陶小荣

Identification, Distribution and Occurrence of Viruses in the Main Vegetables of China

LIU Yong,LI Fan,LI YueYue,ZHANG SongBai,GAO XiWu,XIE Yan,YAN Fei,ZHANG AnSheng,DAI LiangYing,CHENG ZhaoBang,DING Ming,NIU YanBing,WANG ShengJi,CHE HaiYan,JIANG Tong,SHI XiaoBin,HE ZiFu,WU YunFeng,ZHANG DeYong,QING Ling,YAN WanRong,YANG XueHui,TANG YaFei,ZHENG HongYing,TANG QianJun,ZHANG SongBai,ZHANG DongFang,CAI Li,TAO XiaoRong

表2 主要蔬菜病毒检测引物

Table 2 Primers used for detection of the main vegetable viruses

检测病毒 Virus 引物名称 Primer name 序列 Sequence (5′-3′) 长度 Fragment size (bp) 退火温度 Annealing temperature (℃) AMV AMVdF GTGCGTATAGATGCCGGTTC 900 55 AMVdR GAGCGAATAGGACTTCATACC BBWV2 BBWV2dF AGRTATATGCTTGGGCAAGCGCATG 490 57 BBWV2dR CATGAACATTCCCCATCTCCACGTG BCMV BCMVdF CAGCCTTCAATGGAGTTATAACAAT 533 53 BCMVdR ATCTTCTGATTCCAAGTACTGAATTG BYMV BYMVdF GGTGAATGGACAATGATGGATG 550 55 BYMVdR CCTCACTAACTGCTTACCCTG CCYV CCYVdF AGAACATGATCAAGTCGTGAGTC 792 53 CCYVdR GGTAGGAATGAACTCAGTGTCG CGMMV CGMMVdF ATGGCTTACAATCCGATCAC 481 50 CGMMVdR CTAAGCTTTCGAGGTGGTAGC ChiVMV ChiVMVdF GGAAARGCNCCNTAYAT 790 52 ChiVMVdR CGCGCTAATGACATATCGGT CMV CMVCPuF TCTCATGGATGCTTCTCCGCG 760 55 CMVCPuR CCGTAAGCTGGATGGACAACC CMVI CMVIdF1 CCGAAGTAACCCAYGGTCGT 969 55 CMVIdR1 GATTTGTCCATGACTCGACTC CMVII CMVIIdF2 CGCGAGTTAGCGTTTAGTTGT 762 55 CMVIIdR2 TTAACGTCTTCGGACGCCG MPV MPVdF GACCGTTCTCACCTTGACG 924 55 MPVdR CAGTGCAAGTAACCCACATGC MYSV MYSVdF TGACGTCATAATTTCACTAGCAG 588 52 MYSVdR GTCAACTACCTTTACACATCTGA PeVYV PeVYVdF CGTGGAAGCGTGCTACTCG 579 57 PeVYVdR CTCATCAGTGAAGACTCGACC PLRV PLRVdF CCAAGCATACGCGAGTTGC 899 55 PLRVdR GTATGCCAACGTAAGCAATAAATC PMMoV PMMoVdF CCTCTTCCGAGAGAATCTGAGAC 703 54 PMMoVdR CGTGTTTCCAAACTTCAGCCAAG PRSV PRSVdF GACCTACAAGCGTGACTTTAC 703 52 PRSVdR CACCCATGCCATGCATCTTTC PSbMV PSbMVdF GCAAGTGGTTTGATGGTRTGG 536 55 PSbMVdR CATGGTGTCAAAGACACACTG PStV PStVdF TCACAAATGGCTATACTGACATAG 647 52 PStVdR TGGCATTCTTGGCTTGAACAGAG 检测病毒 Virus 引物名称 Primer name 序列 Sequence (5′-3′) 长度 Fragment size (bp) 退火温度 Annealing temperature (℃) PVX PVXdF AGTGCGCGAGGTTTACCAATC 790 54 PVXdR GTGGTTTGCCGCGAACGATTC SPFMV SPFMVdF TGAGTAGAGACGAGTGGTGTG 1188 54 SPFMVdR GTAACACCGCCTACTATGTAGTG SPLV SPLVdF GCACTCTCCTCAGCCTTTG 1077 53 SPLVdR TCTGACGAGCATCATATAGTTTCC SPVC SPVCdF GATTTCCAACTTTTCAAGTCGAAC 783 55 SPVCdR AGCTGTTGCTGATGAAGAAATCCC SPVG SPVGdF CCTCTATTGGATCATGCTGTC 515 52 SPVGdR CTTTTAAGAGTCCCTTATGATACGT STV STVdF TGATGGAGGATATCTACTGTCATT 681 52 STVdR ACAAGATGTTTAAAGCCGTGTCC TAV TAVdF ATGGCCCAAAACGGTACGG 657 57 TAVdR TCACACCGGGAGCGTTGAAG TBTV TBTVdF TACCACACCTAAACAGCGTTG 1049 55 TBTVdR CTCATCTCCCGCTAAGTCAG TMGMV TMGMVdF GAGGAAATTGAGGATAATGTAAGTG 700 55 TMGMVdR ACGCCATACCACAGTATACAC TMV TMVdF GATTCGTTTTAAATATGTCTTAC 600 46 TMVdR CTTCGATTTAAGTGGAGGGA ToCV ToCVdF GCTTCCGAAACTCCGTCTTG 439 51 ToCVdR TGTCGAAAGTACCGCCACC ToMMV ToMMVdF CTGGAGAAGACTGGGTCTAG 1193 55 ToMMVdR TTCGGTAAGTTCAATGGGACCT ToMV ToMVdF TCTCAAGAATGTTACACGGGAAG 980 51 ToMVdR CGCATTCTCCGTAATTTTGATC TSWV TSWVdF TCACTGTAATGTTCCATAGCAA 861 52 TSWVdR AGAGCAATYGTGTCAATTTTATTC TuMV TuMVdF TAAACGGAATGTGGGTGATGATGG 377 56 TuMVdR GTCCTCGGTCGTATGCCTTTCC TVDV TVDVdF GCAACAGCGAGACTTTCATCT 357 56 TVDVdR CRTTGCCTTTATAGAGCAGCC WMV WMVdF CATTGAAAATGGAGTGACACTG 640 51 WMVdR GCCAAAACCTGCATC(A\G)CAC ZYMV ZYMVdF CCATACATAGCTGAGACAGCAC 717 55 ZYMVdR CATACCTCGGCATGTATGGTGCC Begmoviruses BegoAFor1 TGYGARGGICCITGYAARGTYCARTC 1200 50 BegoARev1 ATHCCMDCHATCKTBCTITGCAATCC Poleroviruses PoconF GAYTGYTCYGGTTTTGACTGG 1394 57 PocoCPR CGTCTACCTATTTSGGRTTN Potyviruses CIFor GGIVVIGTIGGIWSIGGIAARTCIAC 700 40 CIRev ACICCRTTYTCDATDATRTTIGTIGC Tobamoviruses TobamodF TKGAYGGNGTBCCNGGNTGYGG 880 55 TobamodR ACNGAVTBNABCTGTAATTGCTAT