紫花苜蓿MsGAI的克隆、表达及遗传转化

张涵,王学敏,刘希强,马琳,温红雨,王赞

Cloning Expression Analysis and Transformation of MsGAI Gene from Medicago sativa L

ZHANG Han,WANG XueMin,LIU XiQiang,MA Lin,WEN HongYu,WANG Zan

图5 MsGAI蛋白与其他物种GAI蛋白的系统发育树 TpGAI：红三叶Trifolium pratense,PNY03470.1;PsLA：豌豆Pisum sativum,ABI30654.1;CaGAI：鹰嘴豆Cicer arietinum,XP_004503135.1;MsGAI：紫花苜蓿Medicago sativa;MtGAI：蒺藜苜蓿Medicago truntula,XP_013460591.1;GmGAI：大豆Glycine max,XP_003552980.1;VaGAI：赤豆Vigna angularis,XP_017418978.1;PvGAI：菜豆Phaseolus vulgaris,BAF62637.1;VvGAI：葡萄Vitis vinifera,ARR27421.1;SiGAI：芝麻Sesamum indicum,XP_011097451.1;GhGAI：陆地棉Gossypium hirsutum,XP_016744906.1;PpGAI：沙梨Pyrus pyrifolia,ANJ78486.1;PsGAI：李子Prunus salicina, AQQ12221.1; AtRGL1：拟南芥Arabidopsis thaliana,NC_003070.9;AtGAI：拟南芥Arabidopsis thaliana,NC_003070.9;AtRGA：拟南芥Arabidopsis thaliana,NC_003071.7;ZmDWARF8：玉米Zea mays,NC_024459.2;HvSLN1：青稞Hordeum vulgare,Q8W127.1;TaRHT1：小麦Triticum aestivum,Q9ST59.1。节点上的数值表示自举值,标尺上的数字表示遗传距离Numbers at node indicates bootstrap value derived from 1000 replicates. The scale bar indicates genetic distance

Fig. 5 Phylogenetic tree of MsGAI and other GAI proteins