紫花苜蓿MsGAI的克隆、表达及遗传转化

张涵,王学敏,刘希强,马琳,温红雨,王赞

Cloning Expression Analysis and Transformation of MsGAI Gene from Medicago sativa L

ZHANG Han,WANG XueMin,LIU XiQiang,MA Lin,WEN HongYu,WANG Zan

表3 PlantCARE启动子预测结果

Table 3 Promoter prediction of PlantCARE

顺式作用元件 Cis-elements 序列 Sequence (5'-3') 功能 Function 数量 No. 3-AF1 binding site AAGAGATATTT 光响应元件 Light responsive element 1 A-box CCGTCC 顺式作用调控元件 Cis-acting regulatory element 2 ACE AAAACGTTTA 光响应顺式元件 Cis-acting element involved in light responsiveness 1 ARE TGGTTT 厌氧诱导必需元件 Cis-acting regulatory element essential for the anaerobic induction 2 ATCT-motif AATCTAATCT 参与光响应的保守DNA模块的部分元件 Part of a conserved DNA module involved in light responsiveness 2 BOX-4 ATTAAT 参与光反应元件 Part of a conserved DNA module involved in light responsiveness 3 Box-W1 TTGACC 真菌诱导子反应元件 Fungal elicitor responsive element 1 CAAT-box CAAAT 启动子和增强子区域中常见的顺式作用元件 Common cis-acting element in promoter and enhancer regions 47 CCAAT-box CAACGG 结合位点MYBHv1 binding site MYBHv1 1 CCGTCC-box CCGTCC 与分生组织特异性激活相关的顺式调控元件 Cis-acting regulatory element related to meristem specific activation 2 GAG-motif AGAGAGT 部分光响应元件 Part of a light responsive element 1 GT1-motif GGTTAA 光响应元件 Light responsive element 1 HSE AAAAAATTTC 热应激响应顺式元件 Cis-acting element involved in heat stress responsiveness 1 LAMP-element CCAAAACCA 部分光响应元件 Part of a light responsive element 1 LTR CCGAAA 参与低温响应的元件 Cis-acting element involved in low-temperature responsiveness 1 MBS TAACTG 参与干旱诱导MYB结合位点 MYB binding site involved in drought-induction 1 MRE AACCTAA 参与光反应的MYB结合位点 MYB binding site involved in light responsiveness 1 Skn-1_motif GTCAT 胚乳表达所需的顺式作用调控元件 Cis-acting regulatory element required for endosperm expression 2 TATA-box ATTATA 在转录起始的-30附近的核心启动子元件 Core promoter element around -30 of transcription start 74 chs-CMA2a GCAATTCC 部分光响应元件 Part of a light responsive element 1 circadian CAANNNNATC 参与昼夜节律顺行调控元件 Cis-acting regulatory element involved in circadian control 3