紫花苜蓿MsGAI的克隆、表达及遗传转化

张涵,王学敏,刘希强,马琳,温红雨,王赞

Cloning Expression Analysis and Transformation of MsGAI Gene from Medicago sativa L

ZHANG Han,WANG XueMin,LIU XiQiang,MA Lin,WEN HongYu,WANG Zan

表1 试验中所用引物序列

Table 1 Sequence of primers used in the experiment

引物名称 Primers 序列 Sequence of primers (5′-3′) 用途 Application MsGAI F: AAACTTCAACCCATAAACTC 基因克隆 Gene cloning R: ACTTAAGGGTACCCTGAG 121-MsGAI F: TGCTCTAGAATGAAGAGAGAACACCA pBI121载体构建 Vector construction R: CGCGGATCCTCACTTGGACTCATTTTG Ms_Actin F: CAAAAGATGGCAGATGCTGAGGAT 内参基因 Internal control R: CATGACACCAGTATGACGAGGTCG QGAI F: CCACCACCTTAACAGCAGCA 荧光定量 Real-time PCR R: GAGCACTACCCATAACCATCTC M13 F: GTAAAACGACGGCCAGT 亚克隆引物 The primers for subcloning R: CAGGAAACAGCTATGAC 35s F: GGTGGCTCCTACAAATGCCA pBI121载体构建 Vector construction R: GAAACGCAGCACGATACGC Promoter F: GGTACACGCTAAGACGCTAC 启动子克隆 Promoter cloning R: TTGCTGCTGTTAAGGTGG