猪链球菌4型转录调控因子GalR的生物学特性

孙珂,祝昊丹,何孔旺,王丹丹,周俊明,俞正玉,吕立新,倪艳秀

Biological Characteristics of Transcriptional Regulator GalR in Streptococcus suis Serotype 4

Ke SUN,HaoDan ZHU,KongWang HE,DanDan WANG,JunMing ZHOU,ZhengYu YU,LiXin LÜ,YanXiu NI

表1 引物序列

Table 1 Primer sequences

引物名称 Primer name 引物序列 Primers sequence (5′→3′) 酶切位点 Restriction sites L1 AAGCTT CCATACAACTCTTCTGCG HindⅢ L2 GTCGAC CAGCTCTTCACTCTCCGT SalⅠ R1 GGATCC CAGCTAGTTATACGGGAG BamHⅠ R2 GAGCTC GGCGAGTAACTACTTCAC SacⅠ C1 GTCGAC CACCGAACTAGAGCTTGATG SalⅠ C2 GGATCC TAATTCGATGGGTTCCGAGG BamHⅠ I1 TTCAACTTGGCACAGGAC I2 CGTGATGGCTGTATCGTT O1 TTTTTCACATTCCGCACG O2 GTTTTGAAGGAAATGCCCA