顶空固相微萃取-气相色谱-质谱联用法分析 欧李果实挥发性成分

李晓颍,王海静,徐宁伟,曹翠玲,刘建珍,武春成,张立彬

Analysis of Volatile Components in Cerasus Humilis (Bge.) Sok by Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry

XiaoYing LI,HaiJing WANG,NingWei XU,CuiLing CAO,JianZhen LIU,ChunCheng WU,LiBin ZHANG

表1 欧李果实挥发性成分及其含量

Table 1 The volatiles and contents of Cerasus humilis (Bge.) Sok

序号 No. 挥发性成分 Volatile 分子式 Formula 分子量 Mass 保留时间 Retention time (min) 含量 Content (μg·kg-1) 保留指数 RI 鉴定依据 Identification Cal. Ref. 醇类 Alcohol 1 2-乙基-己醇 2-Ethyl-hexanol C8H18O 130 20.30 0.02 1034 1035 MS, RI 2 辛醇 Octanol C8H18O 130 23.64 0.03 1083 1087 MS, RI 3 壬醇 Nonanol C9H20O 144 29.12 0.28 1172 1171 MS, RI 4 (Z)-3-己烯-1-醇 (Z)-3-Hexen-1-ol C6H12O 100 8.87 0.01 855 855 MS, RI 5 苯乙醇 Phenylethyl alcohol C8H10O 122 26.05 0.15 1126 1121 MS, RI 芳香类 Arenes 6 邻二甲苯 o-Xylene C8H10 106 9.60 0.06 870 879 MS, RI 7 萘 Naphthalene C10H8 128 29.23 0.02 1174 1170 MS, RI 8 2-甲基萘 2-Methylnaphthalene C11H10 142 33.81 0.03 1289 1289 MS, RI 酸类 Acids 9 苯甲醛 Benzaldehyde C7H6O 106 14.96 0.12 956 961 MS, RI 10 壬醛 Nonanal C9H18O 142 25.62 0.16 1117 1107 MS, RI 11 (E)-3-甲基-4-癸烯酸 (E)-3-Methyl-4-decenoic acid C11H20O2 184 37.59 0.06 1413 1415 MS, RI 萜类 Terpenoids 12 β-芳樟醇 β-Linalool C10H18O 154 25.35 0.24 1111 1103 MS, RI 13 α-法呢烯 α-Farnesene C15H24 204 39.63 0.1 1512 1512 MS, RI 烷类 Alkyls 14 2-壬酮 2-Nonanone C9H18O 142 24.93 0.05 1102 1104 MS, RI 15 十二烷 Dodecane C12H26 170 30.25 0.48 1200 1200 MS, RI 16 十三烷 Tridecane C13H28 184 34.24 0.61 1300 1300 MS, RI 17 2-甲基-十三烷 2-Methyl-tridecane C14H30 198 36.36 0.04 1369 1362 MS, RI 18 法呢烷 Farnesane C15H32 212 36.72 0.08 1381 1378 MS, RI 19 十四烷 Tetradecane C14H30 198 37.33 0.32 1400 1400 MS, RI 20 正辛基环己烷 Octylcyclohexane C14H28 196 38.39 0.10 1451 1451 MS, RI 21 5-甲基-十四烷 5-Methyl-tetradecane C15H32 212 38.51 0.07 1456 1454 MS, RI 22 4-甲基-十四烷 4-Methyl-tetradecane C15H32 212 38.62 0.11 1462 1460 MS, RI 23 4,11-二甲基-十四烷 4,11-Dimethyl-tetradecane C16H34 226 38.70 0.85 1465 1464 MS, RI 24 3-甲基-十四烷 3-Methyl-tetradecane C15H32 212 38.87 0.14 1473 1470 MS, RI 25 十五烷 Pentadecane C15H32 212 39.44 1.31 1500 1500 MS, RI 26 5-丙基-十三烷 5-Propyl-tridecane C16H34 226 39.69 0.16 1515 1516 MS, RI 27 6-甲基-十五烷 6-Methyl-pentadecane C16H34 226 40.29 0.06 1551 1551 MS, RI 28 壬基环已烷 n-Nonylcyclohexane C15H30 210 40.37 0.22 1556 1556 MS, RI 29 4-甲基-十五烷 4-Methyl-pentadecane C16H34 226 40.45 0.04 1560 1557 MS, RI 30 2-甲基-十五烷 2-Methyl-pentadecane C16H34 226 40.53 0.15 1566 1564 MS, RI 序号 No. 挥发性成分 Volatile 分子式 Formula 分子量 Mass 保留时间 Retention time (min) 含量 Content (μg·kg-1) 保留指数 RI 鉴定依据 Identification Cal. Ref. 31 3-甲基-十五烷 3-Methyl-pentadecane C16H34 226 40.65 0.12 1573 1571 MS, RI 32 十六烷 Hexadecane C16H34 226 41.11 0.36 1600 1600 MS, RI 33 2,6,10-三甲基-十五烷 2,6,10-Trimethyl-pentadecane C18H38 254 41.84 0.17 1643 1649 MS, RI 酯类 Esters 34 乙酸乙酯 Ethyl acetate C4H8O2 88 2.95 0.32 659 634 MS 35 己酸乙酯 Ethyl hexanoate C8H16O2 144 18.14 0.11 1003 1001 MS, RI 36 乙酸叶醇酯 (Z)-3-Hexenyl acetate C8H14O2 142 18.63 0.25 1010 1007 MS, RI 37 乙酸己酯 Hexyl acetate C8H16O2 144 19.15 0.66 1017 1017 MS, RI 38 苯甲酸甲酯 Methyl benzoate C8H8O2 136 24.87 0.05 1101 1093 MS, RI 39 异丁酸叶醇酯 (Z)-3-Hexenyl isobutyrate C10H18O2 170 27.79 0.21 1138 1145 MS, RI 40 丁酸己酯 Hexyl butanoate C10H20O2 172 28.05 0.10 1145 1148 MS, RI 41 己酸异丁酯 Isobutyl hexanoate C10H20O2 172 28.16 0.07 1147 1148 MS, RI 42 乙酸苯甲酯 Benzyl acetate C9H10O2 150 28.69 0.14 1161 1161 MS, RI 43 苯甲酸乙酯 Ethyl benzoate C9H10O2 150 28.92 3.25 1167 1170 MS, RI 44 4-辛烯酸乙酯 Ethyl 4-octenoate C10H18O2 170 29.87 0.04 1190 1191 MS, RI 45 辛酸乙酯 Ethyl octanoate C10H20O2 172 30.22 0.20 1199 1201 MS, RI 46 乙酸辛酯 Octyl acetate C10H20O2 172 30.80 0.04 1214 1213 MS, RI 47 异戊酸叶醇酯 cis-3-Hexenyl isovalerate C11H20O2 184 31.56 0.42 1233 1235 MS, RI 48 异戊酸己酯 Hexyl 2-methylbutyrate C11H22O2 186 31.74 0.24 1237 1239 MS, RI 49 2-辛烯酸乙酯 Ethyl (E)-2-octenoate C10H18O2 170 32.12 0.04 1247 1246 MS, RI 50 己酸异戊酯 Isoamyl hexanoate C11H22O2 186 32.23 0.05 1250 1251 MS, RI 51 乙酸苯乙酯 Phenethyl acetate C10H12O2 164 32.47 1.16 1256 1256 MS, RI 52 辛酸丙酯 Propyl octanoate C11H22O2 186 33.96 0.05 1293 1292 MS, RI 53 壬酸乙酯 Ethyl nonanoate C11H22O2 186 34.15 0.05 1298 1298 MS, RI 54 乙酸壬酯 Nonyl acetate C11H22O2 186 34.73 0.08 1316 1314 MS, RI 55 乙酸橙花酯 Neryl Acetate C12H20O2 196 36.95 0.03 1388 1372 MS, RI 56 (E)-己酸-2-己烯酯 (E)-2-Hexenyl hexanoate C12H22O2 198 37.10 0.14 1393 1391 MS, RI 57 癸酸乙酯 Ethyl caprate C12H24O2 200 37.25 0.21 1398 1398 MS, RI 58 (Z)-庚酸-3-己烯酯 (Z)-3-Hexenyl heptanoate C13H24O2 212 39.08 0.13 1483 1489 MS, RI 59 庚酸丁酯 Butyl heptanoate C11H22O2 186 39.17 0.07 1488 1490 MS, RI 60 (Z)-3-辛酸己烯酯 (Z)-3-Hexenyl octanoate C14H26O2 226 40.80 0.78 1582 1588 MS, RI 61 辛酸己酯 Hexyl octanoate C14H28O2 228 40.86 0.54 1585 1582 MS, RI 62 月桂酸乙酯 Ethyl laurate C14H28O2 228 41.05 0.17 1596 1597 MS, RI 63 软脂酸乙酯 Ethyl palmitate C18H36O2 284 46.14 0.09 1900 1914 MS, RI