‘南通小方柿’赤霉素不敏感基因DkGAI2的克隆与功能分析

蒋梦婷,朱宁,龚洪泳,侯应军,余心怡,渠慎春

Cloning and Function Analysis of Gibberellin Insensitive DkGAI2 Gene in Nantongxiaofangshi (Diospyros kaki Linn. cv. nantongxiaofangshi)

MengTing JIANG,Ning ZHU,HongYong GONG,YingJun HOU,XinYi YU,ShenChun QU

表1 ‘南通小方柿’DkGAI2克隆、表达及功能分析所用引物

Table 1 Primers used for molecular cloning, expression and functional analysis of DkGAI2

引物名 Primer name 引物序列 Primer sequence (5′-3′) 用途 Usage DkGAI2-F CACAGCTCGTCCAATCTCCA 中间片段扩增 Intermediate fragment amplification DkGAI2-R CTTCATCTCCTTCACCGCCA DkGAI2 3′ GSP-1 CTTCTCCGACCTCCTCCAA 3′端扩增 3′-end amplification DkGAI2 3′ GSP-2 CTGGATGCGTCAATGCTGG DkGAI2 5′ GSP-1 CTTTGGCACTGGAGGAGGTCATG 5′端扩增 5′-end amplification DkGAI2 5′ GSP-2 GGTGCTGCGGAGGAAGTGGGTTG DkGAI2-ORF-F ATGAAGCGCGACCGCCGCA ORF扩增 ORF amplification DkGAI2-ORF-R TCACCTCTGGTGGGTCTGGA DkGAI2-YG-F CGTCAATGCTGGATCTTC qRT-PCR Quantitative RT-PCR DkGAI2-YG-R CTTCACCGCCAATAACAC NtTubulin-F AGATGTTCCGTCGTGTCAGTG 烟草内参基因 Reference gene in tobacco NtTubulin-R TGCTTCCTCTTCATCCTCATATCC Dkactin-F GCCATCATTAATTGGAATGGAAGC 柿内参基因 Reference gene in persimmon Dkactin-R GTGCCACAACCTTGATCTTCA