柑橘CitPG34的克隆、定位与表达分析

葛廷,黄雪,谢让金

Cloning, Subcellular Localization and Expression Analysis of CitPG34 in Citrus

Ting GE,Xue HUANG,RangJin XIE

表1 本试验所用引物

Table 1 Primers used in this study

引物名称 Primers name 引物序列 Sequence of primers (5′→3′) 目的 Purpose OE-CitPG34-F GCTTTGAAACCATGTCAACATC 基因全长克隆 Full-length gene cloning OE-CitPG34-R TAGGAACTGCCTCTAAGTAAGC CitPG34-F ATTAGCATCCGTAGATAGCC 实时荧光定量 Real-time PCR CitPG34-R CACAGTCATCACCAGTCCC P- CitPG34-F TGATTACGCCAAGCTTACCGTCTATGCTACTGATGTGA 启动子克隆 Promoter cloning P- CitPG34-R AGGGACTGACCACCCTGATGTTGACATGGTTTCAAAGC Cit-Actin-F CCCCATCGTTACCGTCCAG 内参基因 Actin gene Cit-Actin-R CGCCTTGCCAGTTGAATATCC Sub-CitPG34-F GGGGGACTCTTGACCATGTCAACATCAATGTCTTTGCC 亚细胞定位 Subcellular localization sub-CitPG34-R CTAGTCAGATCTACCATGACACCAGAACCACCACCAGA ACCAGTCTTCAAGCAACTATTTGGAAG pCAMBIA1302-F CCTTCACCCTCTCCACTGACAG 亚细胞定位 Subcellular localization pCAMBIA1302-R CCTTCGCAAGACCCTTCCTCTA