近零磁场下灰飞虱转录表达分析稳定性内参基因筛选

刘凡奇,万贵钧,曾路影,李春绪,潘卫东,陈法军

Selection of Stable Internal Reference Genes for Transcript Expression Analyses in Laodelphax striatellus Under Near-Zero Magnetic Field

FanQi LIU,GuiJun WAN,LuYing ZENG,ChunXu LI,WeiDong PAN,FaJun CHEN

表3 利用geNorm、NormFinder、BestKeeper和RefFinder分析灰飞虱短翅雄成虫中各内参基因稳定性

Table 3 The expression stability of the candidate reference genes in brachypterous male adults of L. striatellus calculated by geNorm, NormFinder, BestKeeper and RefFinder

排序 Rank	geNorm		NormFinder		BestKeeper		RefFinder
排序 Rank	基因 Gene	稳定值 Stability value	基因 Gene	稳定值 Stability value	基因 Gene	稳定值 Stability value	基因 Gene	稳定值 Stability value
1	α2TUB	0.012	α2TUB	0.042	Actin1	1.07	α2TUB	1.32
2	RPL9	0.013	Actin1	0.049	RPL9	1.10	Actin1	2.00
3	α1TUB	0.013	RPL9	0.053	α2TUB	1.10	RPL9	2.38
4	RPS11	0.014	GAPDH	0.058	UBI	1.10	α1TUB	3.41
5	RPS15	0.014	α1TUB	0.069	α1TUB	1.11	UBI	5.60
6	Actin1	0.014	RPL8	0.061	GAPDH	1.14	GAPDH	6.00
7	UBI	0.016	RPS15	0.068	ARF2	1.15	RPS11	6.47
8	GAPDH	0.017	UBI	0.069	RPS15	1.16	RPS15	8.24
9	RPL8	0.019	RPS11	0.103	RPS11	1.16	RPL8	8.74
10	ARF2	0.025	EF-1α	0.125	RPL8	1.16	ARF2	9.82
11	EF-1α	0.026	ARF2	0.154	EF-1α	1.16	EF-1α	10.24