近零磁场下灰飞虱转录表达分析稳定性内参基因筛选

刘凡奇,万贵钧,曾路影,李春绪,潘卫东,陈法军

Selection of Stable Internal Reference Genes for Transcript Expression Analyses in Laodelphax striatellus Under Near-Zero Magnetic Field

FanQi LIU,GuiJun WAN,LuYing ZENG,ChunXu LI,WeiDong PAN,FaJun CHEN

表2 利用geNorm、NormFinder、BestKeeper和RefFinder分析灰飞虱短翅雌成虫中各内参基因稳定性

Table 2 The expression stability of the candidate reference genes in brachypterous female adults of L. striatellus calculated by geNorm, NormFinder, BestKeeper and RefFinder

排序 Rank	geNorm		NormFinder		BestKeeper		RefFinder
排序 Rank	基因 Gene	稳定值 Stability value	基因 Gene	稳定值 Stability value	基因 Gene	稳定值 Stability value	基因 Gene	稳定值 Stability value
1	EF-1α	0.171	EF-1α	0.046	UBI	1.05	EF-1α	2.11
2	RPL9	0.173	RPL9	0.046	RPS11	1.07	RPL9	2.21
3	ARF2	0.175	ARF2	0.056	α2TUB	1.10	UBI	3.34
4	RPS15	0.176	α1TUB	0.059	EF-1α	1.11	RPS15	3.36
5	UBI	0.188	RPS15	0.061	Actin1	1.11	ARF2	3.95
6	RPS11	0.196	UBI	0.073	RPL9	1.12	RPS11	4.56
7	α2TUB	0.208	RPS11	0.078	α1TUB	1.13	α2TUB	5.66
8	α1TUB	0.223	α2TUB	0.100	RPS15	1.14	α1TUB	7.44
9	GAPDH	0.237	GAPDH	0.128	ARF2	1.16	Actin1	7.75
10	Actin1	0.248	Actin1	0.129	RPL8	1.21	GAPDH	9.72
11	RPL8	0.264	RPL8	0.137	GAPDH	1.22	RPL8	10.74