近零磁场下灰飞虱转录表达分析稳定性内参基因筛选

刘凡奇,万贵钧,曾路影,李春绪,潘卫东,陈法军

Selection of Stable Internal Reference Genes for Transcript Expression Analyses in Laodelphax striatellus Under Near-Zero Magnetic Field

FanQi LIU,GuiJun WAN,LuYing ZENG,ChunXu LI,WeiDong PAN,FaJun CHEN

表1 内参基因引物信息

Table 1 Primer information of reference genes

引物 Primer 序列 Sequence (5′ to 3′) 扩增效率 Amplification efficiency (%) GenBank编号 GenBank number 描述 Description LSACT1-F CGTGACTTGACCGACTACCT 101.49 KC683802.1 Actin1 LSACT1-R GCACAGCTTCTCCTTGATGTCT LSARF2-F AATCAAAATAAAGCATCGCCCAC 100.44 JF728807.1 ADP ribosylation factor2 LSARF2-R AGCAGCCCCATTATTTAACACGA LSα1TUB-F TCCGAGAAAGCATACCACGAACA 100.58 AY508717.1 Alpha 1-tubulin LSα1TUB-R TCCACGGTACAACATGCAACAAG LSα2TUB-F GTTCGCCATCTACCCTGCTC 100.02 AY550922.1 Alpha 2-tubulin LSα2TUB-R CAGATGTCGTAGATAGCCTCATTGT LSEF-1α-F CTCTGCTCGCCTTCACACTC 99.98 N/A Elongation factor 1 alpha LSEF-1α-R GCCGGGTTGTAGCCAATCTTC LSGAPDH-F GCCAGTGCCCAATGTATCAGTC 100.26 HQ385974.1 Glyceraldehyde-3-phosphate dehydrogenase LSGAPDH-R AGACGACCTCTTCCTCGGTG LSRPL8-F CAACTATGCCACTGTCATTGCT 100.51 HQ385976.1 Ribosomal protein L8 LSRPL8-R CGCCACAATGCCAACCATC LSRPL9-F TGTGACCACCGAAAACAACTCT 100.24 JF728806.1 Ribosomal protein L9 LSRPL9-R TCGTCCTTCTGCTTTGTCGAGT LSRPS11-F GGCACAAGCTACCGCATTAACAT 100.05 N/A Ribosomal protein S11 LSRPS11-R GCCTGTTCCTCTGACAACTACT LSRPS15-F GTTGCCAGCCATTTTATCCAGTC 100.12 N/A Ribosomal protein S15e LSRPS15-R TTGGGTTCTTCGTCTGCCAT LSUBI-F GTTGTTCCAGTGATTGTGCTGT 100.45 N/A Ubiquitin LSUBI-R ACGGCTCCACCTCCAAC