棉铃虫中肠特异性结合蛋白ABCC1对Cry1Ac毒力的影响
陈琳1,魏纪珍2,刘臣1,牛琳琳1,张彩虹1,梁革梅1(
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Effect of Midgut Specific Binding Protein ABCC1 on Cry1Ac Toxicity Against Helicoverpa armigera
CHEN Lin1,WEI JiZhen2,LIU Chen1,NIU LinLin1,ZHANG CaiHong1,LIANG GeMei1()
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图3. 将 pAc-ABCC1转入Sf9细胞并用活化的Cry1Ac蛋白处理后其死亡率
A：PCR检测pAc-ABCC1质粒转入Sf9细胞Confirmation of HaABCC1 expression in cells transfected with pAc-ABCC1 by PCR。1—9：转入pAc-ABCC1质粒的Sf9细胞（3个生物学重复× 3个技术重复）pAc-ABCC1 plasmid transfected into the Sf9 cell line (3 biological replicates × 3 technical replicates);10—18：转入pAc空质粒的Sf9细胞（3个生物学重复× 3个技术重复）pAc empty plasmid transfected into the Sf9 cell line (3 biological replicates × 3 technical replicates)。B：用活化的Cry1Ac蛋白处理转入pAc-ABCC1质粒和转入pAc空质粒的Sf9细胞死亡率The mortality of Sf9 cells exposed to Cry1Ac after transfected with the pAc-ABCC1 or pAc empty plasmid
Fig. 3. Effects of pAc-ABCC1 plasmid transfection into Sf9 cells on the mortality after treated with activated Cry1Ac
