棉铃虫中肠特异性结合蛋白ABCC1对Cry1Ac毒力的影响

陈琳,魏纪珍,刘臣,牛琳琳,张彩虹,梁革梅

Effect of Midgut Specific Binding Protein ABCC1 on Cry1Ac Toxicity Against Helicoverpa armigera

Lin CHEN,JiZhen WEI,Chen LIU,LinLin NIU,CaiHong ZHANG,GeMei LIANG

表1 各RT-PCR、荧光定量RT-PCR及RNAi反应所用引物

Table 1 Listing of primers used in RT-PCR, qRT-PCR and RNAi reactions

引物名称 Primer name 引物序列 Primer sequence (5′- 3′) ABCC1-F TTAATTAACCAGTCCGGCGTCC ABCC1-R ATGTCTTACAATTCTACGCTTGA GAPDH-F CATTGAAGGTCTGATGACCACTGT GAPDH-R CAGAGGGTCCATCCACTGTCTT GAPDH-Probe CACGCCACCATTGCCACCCA β-actin-F GGCCCCGTCCACAATGA β-actin-R CCGATCCATACGGAGTACTTCCT β-actin-Probe ATCAAGATCATCGCGCCCCCAGA ABCC1-F GGCAGCGTGAAAAGAAAGAC ABCC1-R GTGATCACGGACGAGAGGAT ABCC1-Probe CGAAGCTGAGAAAACCGAGACTGGAAG TMD1-EcoRV-F GATATCGCGTTCGGCGGTCAGTTC TMD1-HindIII-R AAGCTTACGTGACATCGAGTTCATCACAATT TMD2-EcoRV-F GATATCTTGATGAGCGTCGGAATCCT TMD2-HindIII-R AAGCTTATGTTTCTACTTCTGATGTCATGCGTAC siRNA-ABCC1 GGAUGUACCUGGUGGGCAUTT siRNA-EGFP GCGUUGGGAAGUCAAGUUUTT ABCC1-PmeI-F GTTTAAACATGTCTTACAATTCTACGCTTG ABCC1-StuI-R AGGCCTTTAATTAACCAGTCCGGCGTCC ABCC1-F’ AGAAGCCTGCCTCCATACTACC ABCC1-R’ AAGAAGCGCAACTGCATAAAC