捻转血矛线虫Hc-FAR-4蛋白的表达特性及其与配体结合能力分析
韦海典,陈学秋,黄艳,时恒枝,周静茹,吴飞,杜爱芳,杨怡(
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The Expression Pattern and Ligand Binding Ability of Hc-FAR-4 Protein of Haemonchus contortus
WEI HaiDian,CHEN XueQiu,HUANG Yan,SHI HengZhi,ZHOU JingRu,WU Fei,DU AiFang,YANG Yi()
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图2. rHc-FAR-4蛋白在E. coli BL21菌株中的诱导表达情况
M：蛋白 Marker;1：pET-30a 空白对照;2：pET-30a-Hc-far-4 未诱导阴性对照;3：pET-30a-Hc-far-4 诱导4 h;4：pET-30a-Hc-far-4 诱导6 h;5：pET-30a-Hc-far-4 诱导8 h
Fig. 2. rHc-FAR-4 protein expressed in E. coli BL21
M: Protein marker; 1: pET-30a empty plasmid; 2: pET-30a-Hc-far-4 were inducted for 0 h; 3: pET-30a-Hc-far-4 were inducted for 4 h; 4: pET- 30a-Hc-far-4 were inducted for 6 h; 5: pET-30a-Hc-far-4 were inducted for 8 h
