鸡线粒体ND1基因变异/异质性及其与性状的关联分析
徐媛媛1,侯玲灵2,姬杰菲1,王焕杰1,李思露1,陈文1,康相涛1,黄艳群1(
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The Variation/Heteroplasmy of Chicken Mitochondrial ND1 Gene and Its Association with Traits
XU YuanYuan1,HOU LingLing2,JI JieFei1,WANG HuanJie1,LI SiLu1,CHEN Wen1,KANG XiangTao1,HUANG YanQun1()
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图5. Sanger测序验证PCR-RFLP检测到的5个异质性个体
1）左侧是利用PCR-RFLP法检测到的5个异质性个体的品种及异质性程度,右侧是通过对其PCR产物进行Sanger测序验证得到的峰图（GS1、GS2—固始鸡,BC1—肉鸡,HC1、HC2—河南斗鸡）。2）红色箭头处为mt.A4589G位点
Fig. 5. Validation of 5 heteroplasmic individuals detected by PCR-RFLP using Sanger sequencing
1) Left is the breeds and heteroplasmic level of the five heterogeneous individuals detected by the PCR-RFLP method, and right is the Sanger sequencing peak map corresponding samples (GS1, GS2 - Gushi chicken, BC1 - Broiler chicken, HC1, HC2 - Henan Cockfighting chicken). 2) The red arrow is the mt.A4589G site
