葡萄miR159s靶基因的鉴定及其应答GA在果实不同组织的调控作用
张文颖1,韩旭1,朱旭东1,解振强2,纠松涛3,黄雨晴1,贾海锋1,房经贵1,王晨1(
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Identification of the Target Genes of VvmiR159s and Their Regulation in Response to GA in Different Tissues of Grape Berry
ZHANG WenYing1,HAN Xu1,ZHU XuDong1,XIE ZhenQiang2,JIU SongTao3,HUANG YuQing1,JIA HaiFeng1,FANG JingGui1,WANG Chen1()
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图2. VvmiR159a/b/c成熟体及前体序列的鉴定
A：VvmiR159a/b/c 3'-miR-RACE和5'-miR-RACE的PCR产物;B：VvmiR159a/b/c前体序列的PCR产物;C：VvmiR159s克隆序列与MiRBase中的同源序列比较;D：VvmiR159s前体的二级茎环结构
Fig. 2. Identification of VvmiR159a/b/c mature and precursor sequences
A: The PCR products of 3’-miR-RACE and 5’-miR-RACE for VvmiR159a/b/c, respectively; B: PCR products of VvmiR159a/b/c primary sequences; C: Comparison of cloned VvmiR159s precise sequences with the homologous sequences in grapes from MiRBase; D: Hairpin structures of VvmiR159s
