葡萄miR159s靶基因的鉴定及其应答GA在果实不同组织的调控作用

张文颖,韩旭,朱旭东,解振强,纠松涛,黄雨晴,贾海锋,房经贵,王晨

Identification of the Target Genes of VvmiR159s and Their Regulation in Response to GA in Different Tissues of Grape Berry

WenYing ZHANG,Xu HAN,XuDong ZHU,ZhenQiang XIE,SongTao JIU,YuQing HUANG,HaiFeng JIA,JingGui FANG,Chen WANG

表1 引物序列及用途

Table 1 Sequence and use of primers

引物名称 Primer name 引物序列 Primer Sequence 引物用途 Use VvmiR159a-F CCATGGAATCTTTTTCCGGACACTACAACA VvmiR159a前体序列的扩增 Amplification of VvmiR159a precursor VvmiR159a-R ACTAGTATCCCACCCGGATTTTGAAATAA VvmiR159b-F CCATGGTGTTATTGGGTTTGGGTCATCTCA VvmiR159b前体序列的扩增 Amplification of VvmiR159b precursor VvmiR159b-R ACTAGTACCCAGATTAACAATACAAAAGGTC VvmiR159c-F CCATGGCAGTCAGTAATGCAAGCCACA VvmiR159c前体序列的扩增 Amplification of VvmiR159c precursor VvmiR159c-R ACTAGTAGCTGCAGTTTTGTTGGGTTT Actin-F TACAATTCCATCATGAAGTGTGATG Actin 内参引物 Actin primer Actin-R TTAGAAGCACTTCCTGTGAACAATG VvmiR159a qRT-F CTTCTCCGAGGTCATGCACA VvmiR159a 定量RT-PCR引物 Real-time PCR of VvmiR159a VvmiR159a qRT-R ATGGGAGCTCCTTTACGCTC VvmiR159b qRT-F TTGCCATTCCAACACTCATC VvmiR159b 定量RT-PCR引物 Real-time PCR of VvmiR159b VvmiR159b qRT-R TTTCTGGCCTTGGAGTGAAG VvmiR159c qRT-F GCTCCCTTCAATCCAAACAA VvmiR159c 定量RT-PCR引物 Real-time PCR of VvmiR159c VvmiR159c qRT-R TTATGGATCCCACAGCCCTA VvGAMYB qRT-F GAAATGGAATCCCCCTGTTT VvGAMYB 定量RT-PCR引物 Real-time PCR of VvGAMYB VvGAMYB qRT-R TCCTTGTTGGTAGGGTCAGG VvMYB48 qRT-F TTCTCAGGAACTGGGGAATG VvMYB48 定量RT-PCR引物 Real-time PCR of VvMYB48 VvMYB48 qRT-R AAAGAAGCTGTCCGGAGTCA VvAIX15 qRT-F GTGCCCAAAGGCTATGTTCC VvAIX15 定量RT-PCR引物 Real-time PCR of VvAIX15 VvAIX15 qRT-R TCTTCCCTGCATGGGATTGT VvGRAS-l qRT-F TTGGAGGCCTTAGAAGGGGA VvGRAS-l 定量RT-PCR引物 Real-time PCR of VvGRAS-l VvGRAS-l qRT-R ACAGTGAGCTTTAGGCGAGG