基于种特异性COI标记的西花蓟马温室品系和羽扇豆品系一步双重快速鉴定技术
张蓉1,王玉生1,杨丽梅3,万方浩1,2,张桂芬1,2(
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One-Step Duplex Rapid Identification Technique of Frankliniella occidentalis Greenhouse and Lupin Races Based on Species-Specific COI Marker
ZHANG Rong1,WANG YuSheng1,YANG LiMei3,WAN FangHao1,2,ZHANG GuiFen1,2()
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图6. 西花蓟马品系特异性组合引物TF6/GR32/LR12对温室品系（A）和羽扇豆品系（B）最低检测阈值的测定
Fig. 6. The detection threshold for F. occidentalis greenhouse race (A) and lupin race (B) diluted female adult mtDNA using race-specific combination primers TF6/GR32/LR12
A：温室品系 Greenhouse race;M：标准DNA分子质量 DNA ladder;1—13：18.38×103, 9.19×103, 4.60×103, 2.30×103, 1.15×103, 574.38, 287.19, 143.59, 71.80, 35.90, 17.95, 8.97, and 4.49 pg·μL-1, respectively;14：阴性对照（模板为超纯水）Negative control (ultrapure water)。B：羽扇豆品系 Lupin race;M：标准DNA分子质量 DNA ladder;1—13：18.81×103, 9.41×103, 4.70×103, 2.35×103, 1.18×103, 587.81, 293.91, 146.95, 73.48, 36.74, 18.37, 9.18, and 4.59 pg·μL-1, respectively;14：阴性对照（模板为超纯水）Negative control (ultrapure water)
