基于种特异性COI标记的西花蓟马温室品系和羽扇豆品系一步双重快速鉴定技术
张蓉1,王玉生1,杨丽梅3,万方浩1,2,张桂芬1,2(
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One-Step Duplex Rapid Identification Technique of Frankliniella occidentalis Greenhouse and Lupin Races Based on Species-Specific COI Marker
ZHANG Rong1,WANG YuSheng1,YANG LiMei3,WAN FangHao1,2,ZHANG GuiFen1,2()
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图4. 西花蓟马品系特异性组合引物TF6/GR32/LR12对温室品系和羽扇豆品系及其他14种田间常见蓟马的PCR扩增
Fig. 4. Amplification pattern of DNA of F. occidentalis greenhouse and lupin races, and other 14 common thrips species in the field using race-specific combination primers TF6/GR32/LR12
M：标准DNA分子质量DNA ladder;1：西花蓟马温室品系F. occidentalis greenhouse race;2：西花蓟马羽扇豆品系F. occidentalis lupin race;3：花蓟马F. intonsa;4：禾花蓟马F. tenuicornis;5：黄胸蓟马T. hawaiiensis;6：大蓟马T. major;7：棕榈蓟马T. palmi;8：烟蓟马T. tabaci;9：普通大蓟马M. usitatus;10：豆喙蓟马M. glycines;11：草木樨近绢蓟马S. melilotus;12：蔗腹齿蓟马F. serrata;13：稻简管蓟马H. aculeatus;14：榕端宽管蓟马M. jordani;15：榕母管蓟马G. ficorum;16：横纹蓟马A. fasciatus;17：阴性对照（模板为超纯水）Negative control (ultrapure water)
