基于种特异性COI标记的西花蓟马温室品系和羽扇豆品系一步双重快速鉴定技术
张蓉1,王玉生1,杨丽梅3,万方浩1,2,张桂芬1,2(
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One-Step Duplex Rapid Identification Technique of Frankliniella occidentalis Greenhouse and Lupin Races Based on Species-Specific COI Marker
ZHANG Rong1,WANG YuSheng1,YANG LiMei3,WAN FangHao1,2,ZHANG GuiFen1,2()
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图3. 西花蓟马温室品系（A）和羽扇豆品系（B）一步双重特异性组合引物TF6/GR32/LR12 PCR扩增的退火温度优化
Fig. 3. Optimization of annealing temperature of the one-step race-specific combination primers TF6/GR32/LR12 for DNA of F. occidentalis greenhouse (A) and lupin (B) races
M：标准DNA分子质量 DNA ladder;1—10：退火温度分别为 Annealing temperatures are 36, 38, 40, 42, 44, 46, 48, 50, 52, 54℃, respectively;11：阴性对照（模板为超纯水）Negative control (ultrapure water)
