基于种特异性COI标记的西花蓟马温室品系和羽扇豆品系一步双重快速鉴定技术
张蓉1,王玉生1,杨丽梅3,万方浩1,2,张桂芬1,2(
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One-Step Duplex Rapid Identification Technique of Frankliniella occidentalis Greenhouse and Lupin Races Based on Species-Specific COI Marker
ZHANG Rong1,WANG YuSheng1,YANG LiMei3,WAN FangHao1,2,ZHANG GuiFen1,2()
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图2. 西花蓟马一步双重品系特异性引物TF6/GR32/LR12不同比例组合对温室品系（A）和羽扇豆品系（B）的扩增
Fig. 2. Amplification pattern of different ratios of race-specific combination primers TF6/GR32/LR12 on DNA of F. occidentalis greenhouse (A) and lupin (B) races
M：标准DNA分子质量 DNA ladder;1—11：TF6/GR32/LR12比例分别为The ratios of TF6/GR32/LR12 are 1.0/0/1.0, 1.0/0.1/0.9, 1.0/0.2/0.8, 1.0/0.3/0.7, 1.0/0.4/0.6, 1.0/0.5/0.5, 1.0/0.6/0.4, 1.0/0.7/0.3, 1.0/0.8/0.2, 1.0/0.9/0.1, and 1.0/1.0/0 μL, respectively;12：阴性对照（模板为超纯水）Negative control (ultrapure water)
