基于种特异性COI标记的西花蓟马温室品系和羽扇豆品系一步双重快速鉴定技术
张蓉1,王玉生1,杨丽梅3,万方浩1,2,张桂芬1,2(
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One-Step Duplex Rapid Identification Technique of Frankliniella occidentalis Greenhouse and Lupin Races Based on Species-Specific COI Marker
ZHANG Rong1,WANG YuSheng1,YANG LiMei3,WAN FangHao1,2,ZHANG GuiFen1,2()
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图1. DNA条形码通用型引物LCO1490/HCO2198对西花蓟马温室品系和羽扇豆品系以及其他14种田间常见蓟马的扩增电泳图
Fig. 1. Gel images of PCR amplifications of DNA from F. occidentalis greenhouse and lupin races, and other 14 common thrips species in the field using COI gene universal primers LCO1490/HCO2198
M：标准DNA分子质量 DNA ladder;1：西花蓟马温室品系F. occidentalis greenhouse race;2：西花蓟马羽扇豆品系F. occidentalis lupin race;3：花蓟马F. intonsa;4：禾花蓟马F. tenuicornis;5：黄胸蓟马T. hawaiiensis;6：大蓟马T. major;7：棕榈蓟马T. palmi;8：烟蓟马T. tabaci;9：普通大蓟马M. usitatus;10：豆喙蓟马M. glycines;11：草木樨近绢蓟马S. melilotus;12：蔗腹齿蓟马F. serrata;13：稻简管蓟马H. aculeatus;14：榕端宽管蓟马M. jordani;15：榕母管蓟马G. ficorum;16：横纹蓟马A. fasciatus;17：阴性对照（模板为超纯水）Negative control (ultrapure water)
