基于种特异性COI标记的西花蓟马温室品系和羽扇豆品系一步双重快速鉴定技术

张蓉,王玉生,杨丽梅,万方浩,张桂芬

One-Step Duplex Rapid Identification Technique of Frankliniella occidentalis Greenhouse and Lupin Races Based on Species-Specific COI Marker

Rong ZHANG,YuSheng WANG,LiMei YANG,FangHao WAN,GuiFen ZHANG

表2 西花蓟马品系特异性引物序列及扩增产物

Table 2 The sequence of F. occidentalis race-specific primer and amplification product

品系 Race 引物名称 Primer name 碱基序列 Primer sequence (5′-3′) 扩增片段长度 Fragment length (bp) 温室品系 Greenhouse race TF6/GR32 CGACTTAATAACATAAGATTTT/ GATGTATCTAAGTCTCGGTCT 362 羽扇豆品系 Lupin race TF6/LR12 CGACTTAATAACATAAGATTTT/ GCATAGCATAGATTAGTCCC 541