甘肃省白菜死棵病病原菌鉴定及其融合群检测技术
王朵,谢学文,柴阿丽,石延霞(
),李宝聚()
),李宝聚()
Identification of the Pathogen Causing Cabbage Died in Gansu Province and Detection of Anastomosis Groups
WANG Duo,XIE XueWen,CHAI ALi,SHI YanXia(),LI BaoJu()
),LI BaoJu()
图4. 常规PCR扩增目的片段
Fig. 4. The amplification of R. solani fragment by conventional PCR
M：DNA分子量标准（5 000 bp） DNA marker (5 000 bp);1：阳性对照Positive control;2：感染立枯丝核菌的白菜样品R. solani-infected cabbage sample;3—21：立枯丝核菌融合群AG-3—AG-11菌株、水稻纹枯病菌、双核丝核菌AG-A、小麦纹枯病菌、茄镰孢、尖镰孢、胶孢炭疽菌、瓜果腐霉、终极腐霉、芸薹生链格孢菌和致病疫霉 R. solani AG-3 to AG-11, R. solani AG-1-IA, binulceate Rhizoctonia AG-A, R. cerealis, F. solani, F. oxysporum, C. gloeosporioides, P. aphanidermatum, P. ultimum, A. brassicicola, P. infestans;22：健康白菜样品 Healthy cabbage sample;23：空白对照 Blank control
